{"title":"Screening and performance of efficient lignin-degrading fungal consortium MR","authors":"Chenglong Hu, Guoxiang Zheng, Shengnan Chen, Siyu Wang, Hongying Shi, Dongyu Li, Guohui Qin","doi":"10.1007/s13399-024-05879-6","DOIUrl":null,"url":null,"abstract":"<p>The complex structure of lignocellulosic biomass is a key factor impeding its bioconversion. This study identified three fungi with high lignin-degrading efficiency from decayed wood and established a fungal consortium named MR. Utilizing response surface methodology, the study optimized enzyme production by MR. This resulted in achieving a maximum laccase activity of 668.83 U/L under specific conditions: 68.91 mL working volume, 12.16% inoculum, 22.57 carbon-to-nitrogen ratio, and 6.2 days of incubation. The effectiveness of MR’s hydrolysis was demonstrated by utilizing 10 g/L soybean straw as the sole carbon source, with an 8% inoculum and a 4-day inoculation period. The relative degradation rate of lignin remained at 68.4%. The acetic acid concentration in the hydrolysate reached 209.85 mmol/L, representing over 80% of all volatile fatty acids. These findings suggest that the fungal consortium MR holds significant promise for efficiently converting lignocellulosic biomass into bioenergy and biofuels, offering a potentially sustainable solution to address energy and environmental challenges.</p><h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>\n","PeriodicalId":488,"journal":{"name":"Biomass Conversion and Biorefinery","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomass Conversion and Biorefinery","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s13399-024-05879-6","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENERGY & FUELS","Score":null,"Total":0}
引用次数: 0
Abstract
The complex structure of lignocellulosic biomass is a key factor impeding its bioconversion. This study identified three fungi with high lignin-degrading efficiency from decayed wood and established a fungal consortium named MR. Utilizing response surface methodology, the study optimized enzyme production by MR. This resulted in achieving a maximum laccase activity of 668.83 U/L under specific conditions: 68.91 mL working volume, 12.16% inoculum, 22.57 carbon-to-nitrogen ratio, and 6.2 days of incubation. The effectiveness of MR’s hydrolysis was demonstrated by utilizing 10 g/L soybean straw as the sole carbon source, with an 8% inoculum and a 4-day inoculation period. The relative degradation rate of lignin remained at 68.4%. The acetic acid concentration in the hydrolysate reached 209.85 mmol/L, representing over 80% of all volatile fatty acids. These findings suggest that the fungal consortium MR holds significant promise for efficiently converting lignocellulosic biomass into bioenergy and biofuels, offering a potentially sustainable solution to address energy and environmental challenges.
期刊介绍:
Biomass Conversion and Biorefinery presents articles and information on research, development and applications in thermo-chemical conversion; physico-chemical conversion and bio-chemical conversion, including all necessary steps for the provision and preparation of the biomass as well as all possible downstream processing steps for the environmentally sound and economically viable provision of energy and chemical products.