Effects of protease enzyme supplementation and varying levels of amino acid inclusion on productive performance, egg quality, amino acid digestibility, and economics of egg production in Hy-Line W-36 laying hen from 50 to 70 wk of age

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Applied Poultry Research Pub Date : 2024-06-18 DOI:10.1016/j.japr.2024.100452

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引用次数: 0

Abstract

This study evaluated the effects of reducing total amino acid (AA) & crude protein (CP) level and supplementation of protease on egg production, egg quality, AA digestibility and economics of egg production in W-36 laying hens from 50-70 wk of age. Five hundred seventy-six Hy-Line W-36 hens were housed in 192 cages consisting of 12 reps of 2 side-by side cages containing 3 hens. Each replicate was fed one of 8 diets in a 4 × 2 factorial arrangement of AA&CP levels (85, 90, 95, and 100% of the breeder recommendations) and protease (exclusion or inclusion). Protease was incorporated into the feed at the dosage recommended by the manufacturer, which is 60 grams per metric ton. Corn and soybean meal-based diets were formulated to meet 100% of the breeder's recommendations for the standardized ileal digestibility of Lys, Met, Thr, Trp, TSAA, Ile, and Val. Our study explored the effects of varying levels of AA&CP set at 85, 90, 95, and 100% of the recommendations for the ileal digestibility of Lys, Thr, Trp, TSAA, Ile, and Val, in diets with and without protease supplementation. All diets were supplemented with phytase at 500 phytase units/kg. Data were analyzed using PROC GLM of SAS 9.4 (2019) and the means were separated using Tukey's multiple comparison tests (P < 0.05). Overall, an interaction was observed between AA&CP level and protease supplementation on egg weight (EW; P = 0.003). The main effect of AA&CP level was observed on haugh unit (HU; P < 0.001) and feed cost (P < 0.001) where higher values were observed in 100 and 95% CP level diets as compared to 90 and 85% CP level diets. Similarly, a decrease in the AA&CP level increased the apparent ileal digestibility (AID) of CP (P < 0.001), Lys (P < 0.001), Met (P < 0.001), Thr (P < 0.001), Trp (P < 0.001), Ile (P < 0.001), and Val (P < 0.001). Protease supplementation increased the AID of CP (P = 0.003), Lys (P = 0.001), Thr (P = 0.009), Ile (P < 0.001), and Val (P < 0.001). In conclusion, lower levels of AA&CP supplemented with protease did not negatively affect the egg production, albumen quality and the cost of production in low AA&CP corn and soybean meal-based diets in the Hy-Line W-36 laying hen.

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补充蛋白酶和添加不同水平的氨基酸对 50 至 70 周龄 Hy-Line W-36 型蛋鸡生产性能、鸡蛋质量、氨基酸消化率和产蛋经济性的影响

本研究评估了降低总氨基酸（）和粗蛋白（）水平以及补充蛋白酶对 50-70 周龄 W-36 型蛋鸡产蛋量、蛋品质、AA 消化率和产蛋经济性的影响。576 只 Hy-Line W-36 蛋鸡被饲养在 192 个鸡笼中，其中包括 12 个并排的 2 个鸡笼，每个鸡笼饲养 3 只母鸡。每个重复饲喂 8 种日粮中的一种，日粮的 AA&CP 水平（种鸡推荐值的 85、90、95 和 100%）和蛋白酶（排除或加入）采用 4 × 2 的因子排列。蛋白酶按生产商推荐的剂量（每公吨 60 克）加入饲料中。以玉米和豆粕为基础配制的日粮在 Lys、Met、Thr、Trp、TSAA、Ile 和 Val 的标准化回肠消化率方面达到了饲养员建议的 100%。我们的研究探讨了在添加或不添加蛋白酶的日粮中，将 AA&CP 的水平设定为 Lys、Thr、Trp、TSAA、Ile 和 Val 的回肠消化率建议值的 85、90、95 和 100%的不同效果。所有日粮都添加了植酸酶，添加量为 500 植酸酶单位/千克。数据使用 SAS 9.4（2019 年）的 PROC GLM 进行分析，并使用 Tukey's 多重比较检验（< 0.05）来区分平均值。总体而言，AA&CP 水平和蛋白酶补充量对蛋重存在交互作用 (; = 0.003)。AA&CP水平对蛋重单位（; < 0.001）和饲料成本（ < 0.001）有主效应，与90%和85%CP水平日粮相比，100%和95%CP水平日粮的饲料成本值更高。同样，AA&CP 水平的降低增加了 CP（< 0.001）、Lys（< 0.001）、Met（< 0.001）、Thr（< 0.001）、Trp（< 0.001）、Ile（< 0.001）和 Val（< 0.001）的表观回肠消化率（）。蛋白酶补充剂增加了CP（= 0.003）、Lys（= 0.001）、Thr（= 0.009）、Ile（< 0.001）和Val（< 0.001）的AID。总之，在以玉米和豆粕为基础的低 AA&CP 日粮中，添加蛋白酶的较低 AA&CP 水平不会对 Hy-Line W-36 产蛋鸡的产蛋量、蛋白质量和生产成本产生负面影响。

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来源期刊

Journal of Applied Poultry Research 农林科学-奶制品与动物科学

CiteScore

4.10

自引率

10.50%

发文量

审稿时长

104 days

期刊介绍： The Journal of Applied Poultry Research (JAPR) publishes original research reports, field reports, and reviews on breeding, hatching, health and disease, layer management, meat bird processing and products, meat bird management, microbiology, food safety, nutrition, environment, sanitation, welfare, and economics. As of January 2020, JAPR will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. The readers of JAPR are in education, extension, industry, and government, including research, teaching, administration, veterinary medicine, management, production, quality assurance, product development, and technical services. Nutritionists, breeder flock supervisors, production managers, microbiologists, laboratory personnel, food safety and sanitation managers, poultry processing managers, feed manufacturers, and egg producers use JAPR to keep up with current applied poultry research.