Molecular Characterization and Comparative Transcriptomic Analysis of Hr-Lcyb and Hr-Psy Genes for Carotenoids Synthesis in Sea Buckthorn (Hippophae rhamnoides L.)

IF 3.4 3区农林科学 Q2 ENVIRONMENTAL SCIENCES

Journal of Soil Science and Plant Nutrition Pub Date : 2024-07-03 DOI:10.1007/s42729-024-01891-x

Shazia Arif, Hafiz Muhammad Ahmed, Safira Attacha, Kotb A. Attia, Arif Ahmed Mohammed, Saira Ishaq, Shahid Iqbal Awan, Sajid Fiaz, Waseem Alam, Seung Hwan Yang

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引用次数: 0

Abstract

Sea buckthorn (Hippophae rhamnoides) is an important medicinal plant of Southeast-Asia, enriched with various antioxidant components like β-carotene, alpha-tocopherol, catechin and folacin. Carotenoids are the bioactive substances abundantly present in sea buckthorn berries providing it an enticing yellowish, or orange red color. We performed the phylogenetic analysis, and conserved domain analysis to observe the evolutionary history and functional cites of carotenoids biosynthesis genes respectively. Further relative expression of two carotenoids biosynthesis genes Lycopene β-cyclase (Hr-Lcyb) and phytoene synthase (Hr-Psy) in was conducted in various tissues of sea buckthorn plants. Our results revealed that both the carotenoid synthesis genes Hr-Lcyb and Hr-Psy showed homology with already reported Arabidopsis thaliana genes (AT3G10230 and AT5G17230). Relative expression analysis indicated that expression of Hr-Lcyb and Hr-Psy genes in leaves and floral organs of sea buckthorn was one-fold higher than other tissues. This study will open the door for plant biologists for further characterization of carotenoids synthesis genes and their regulatory processes in specific tissues of carotenoid rich fruit plants.

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沙棘（Hippophae rhamnoides L.）中合成类胡萝卜素的 Hr-Lcyb 和 Hr-Psy 基因的分子特征和转录组比较分析

沙棘（Hippophae rhamnoides）是东南亚的一种重要药用植物，富含多种抗氧化成分，如β-胡萝卜素、α-生育酚、儿茶素和叶酸。类胡萝卜素是沙棘果中含量丰富的生物活性物质，使其呈现诱人的淡黄色或橙红色。我们分别对类胡萝卜素生物合成基因进行了系统进化分析和保守结构域分析，以观察其进化历史和功能特性。我们还进一步研究了两种类胡萝卜素生物合成基因番茄红素β-环化酶（Hr-Lcyb）和植物烯合成酶（Hr-Psy）在沙棘植株不同组织中的相对表达情况。结果表明，类胡萝卜素合成基因 Hr-Lcyb 和 Hr-Psy 与已报道的拟南芥基因（AT3G10230 和 AT5G17230）具有同源性。相对表达分析表明，沙棘叶和花器官中 Hr-Lcyb 和 Hr-Psy 基因的表达量比其他组织高出 1 倍。这项研究将为植物生物学家进一步鉴定类胡萝卜素合成基因及其在富含类胡萝卜素果实植物特定组织中的调控过程打开一扇大门。

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来源期刊

Journal of Soil Science and Plant Nutrition Agricultural and Biological Sciences-Soil Science

CiteScore

5.90

自引率

10.30%

发文量

331

审稿时长

9 months

期刊介绍： The Journal of Soil Science and Plant Nutrition is an international, peer reviewed journal devoted to publishing original research findings in the areas of soil science, plant nutrition, agriculture and environmental science. Soil sciences submissions may cover physics, chemistry, biology, microbiology, mineralogy, ecology, pedology, soil classification and amelioration. Plant nutrition and agriculture submissions may include plant production, physiology and metabolism of plants, plant ecology, diversity and sustainability of agricultural systems, organic and inorganic fertilization in relation to their impact on yields, quality of plants and ecological systems, and agroecosystems studies. Submissions covering soil degradation, environmental pollution, nature conservation, and environmental protection are also welcome. The journal considers for publication original research articles, technical notes, short communication, and reviews (both voluntary and by invitation), and letters to the editor.