Subinhibitory concentrations of nisin enhance virulence gene expression in Staphylococcus aureus and increase mortality in Galleria mellonella.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Letters in Applied Microbiology Pub Date : 2024-07-01 DOI:10.1093/lambio/ovae064

Ramila Cristiane Rodrigues, Cleriane Andre, Maria Cristina Dantas Vanetti

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引用次数: 0

Abstract

Alternative strategies for controlling Staphylococcus aureus and other pathogens have been continuously investigated, with nisin, a bacteriocin widely used in the food industry as a biopreservative, gaining increasing attention. In addition to its antimicrobial properties, bacteriocins have significant effects on genome functionality even at inhibitory concentrations. This study investigated the impact of subinhibitory concentrations of nisin on S. aureus. Culturing in the presence of 0.625 μmol l-1 nisin, led to the increased relative expression of hla, saeR, and sarA, genes associated with virulence while expression of the sea gene, encoding staphylococcal enterotoxin A (SEA), decreased. In an in vivo experiment, Galleria mellonella larvae inoculated with S. aureus cultured in the presence of nisin exhibited 97% mortality at 72 h post-infection, compared to over 40% of larvae mortality in larvae infected with S. aureus. A comprehensive understanding of the effect of nisin on the transcriptional response of virulence genes and the impact of these changes on the virulence of S. aureus can contribute to assessing the application of this bacteriocin in food and medical contexts.

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亚抑制浓度的 nisin 可增强金黄色葡萄球菌毒力基因的表达，并增加 Galleria mellonella 的死亡率。

人们一直在研究控制金黄色葡萄球菌和其他病原体的替代策略，其中作为生物防腐剂广泛应用于食品工业的细菌素--尼生素越来越受到关注。除了抗菌特性外，细菌素即使在抑制浓度下也会对基因组功能产生显著影响。本研究调查了亚抑制浓度的尼生素对金黄色葡萄球菌的影响。在 0.625 μmol l-1 nisin 存在下培养，与毒力相关的 hla、saeR 和 sarA 基因的相对表达量增加，而编码葡萄球菌肠毒素 A（SEA）的 sea 基因的表达量减少。在一项体内实验中，接种了存在 nisin 的金黄色葡萄球菌的 Galleria mellonella 幼虫在感染后 72 小时的死亡率为 97%，而感染了金黄色葡萄球菌的幼虫死亡率超过 40%。全面了解尼生素对毒力基因转录反应的影响以及这些变化对金黄色葡萄球菌毒力的影响，有助于评估这种细菌素在食品和医疗方面的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Letters in Applied Microbiology 工程技术-生物工程与应用微生物

CiteScore

4.40

自引率

4.20%

发文量

225

审稿时长

3.3 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.