Stereospecific reduction of 2'S-configured strigolactones by cowpea OPR3 enzymes.

IF 1.4 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Shota Suzawa, Misa Yamauchi, Masato Homma, Yasuo Yamauchi, Masaharu Mizutani, Takatoshi Wakabayashi, Yukihiro Sugimoto
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引用次数: 0

Abstract

Strigolactones (SLs), plant-derived apocarotenoids, serve dual roles as phytohormones and rhizosphere signaling molecules. While exogenous administration of SLs to plants aids in studying their functions, the metabolic destiny of these administered SLs remains poorly elucidated. Our previous research demonstrated that among synthetic SL GR24 stereoisomers administered to cowpea (Vigna unguiculata), 2'-epi-GR24 undergoes selective reduction at the C-3',4' double bond in its D-ring. In this investigation, we isolated proteins from cowpea roots based on SL reducing activity and identified 12-oxophytodienoate reductase 3 homologs (VuOPR3s) as contributors to this reduction. Enzymatic assays conducted with recombinant proteins revealed that VuOPR3s exhibited a preference for reducing activity toward 2'S-configured SLs, including 2'-epi-GR24. This specificity for 2'S-configured SLs was congruent with that observed for orobanchol produced by cowpea and its stereoisomers. These findings suggest that exogenously administered SLs undergo enzymatic stereoselective reduction, underscoring the importance of considering stereospecificity when interpreting data obtained from SL usage.

豇豆 OPR3 酶对 2'S-configured strigolactones 的立体特异性还原。
人参内酯(SLs)是植物源性类胡萝卜素,具有植物激素和根瘤信号分子的双重作用。虽然向植物外源施用 SLs 有助于研究它们的功能,但对这些施用的 SLs 的代谢去向仍然知之甚少。我们之前的研究表明,在给豇豆(Vigna unguiculata)施用的合成 SL GR24 立体异构体中,2'-epi-GR24 会在其 D 环的 C-3',4'双键处发生选择性还原。在这项研究中,我们根据 SL 还原活性从豇豆根中分离出蛋白质,并确定 12-氧代二烯酸还原酶 3 同源物(VuOPR3s)是这种还原的贡献者。利用重组蛋白进行的酶测定显示,VuOPR3s 对 2'S-configured SLs(包括 2'-epi-GR24 )具有偏好还原活性。这种对 2'S 构建的 SL 的特异性与对豇豆产生的邻苯二酚及其立体异构体的观察结果一致。这些研究结果表明,外源给药的可溶性单体会发生酶促立体选择性还原,从而强调了在解释从可溶性单体使用中获得的数据时考虑立体特异性的重要性。
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来源期刊
Bioscience, Biotechnology, and Biochemistry
Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学
CiteScore
3.50
自引率
0.00%
发文量
183
审稿时长
1 months
期刊介绍: Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).
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