Non-invasive optoacoustic imaging of dermal microcirculatory revascularization in diet-induced obese mice undergoing exercise intervention

IF 7.1 1区医学 Q1 ENGINEERING, BIOMEDICAL

Photoacoustics Pub Date : 2024-06-30 DOI:10.1016/j.pacs.2024.100628

Shan Huang , Hailong He , Robby Zachariah Tom , Sarah Glasl , Pia Anzenhofer , Andre C. Stiel , Susanna M. Hofmann , Vasilis Ntziachristos

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引用次数: 0

Abstract

Microcirculatory dysfunction has been observed in the dermal white adipose tissue (dWAT) and subcutaneous white adipose tissue (scWAT) of obese humans and has been proposed as an early prediction marker for cardio-metabolic disease progression. In-vivo visualization and longitudinal monitoring of microvascular remodeling in these tissues remains challenging. We compare the performance of two optoacoustic imaging methods, i.e. multi-spectral optoacoustic tomography (MSOT) and raster-scanning optoacoustic mesoscopy (RSOM) in visualizing lipid and hemoglobin contrast in scWAT and dWAT in a mouse model of diet-induced obesity (DIO) undergoing voluntary wheel running intervention for 32 weeks. MSOT visualized lipid and hemoglobin contrast in murine fat depots in a quantitative manner even at early stages of DIO. We show for the first time to our knowledge that RSOM allows precise visualization of the dWAT microvasculature and provides quantitative readouts of skin layer thickness and vascular density in dWAT and dermis. Combination of MSOT and RSOM resolved exercise-induced morphological changes in microvasculature density, tissue oxygen saturation, lipid and blood volume content in dWAT and scWAT. The combination of MSOT and RSOM may allow precise monitoring of microcirculatory dysfunction and intervention response in dWAT and scWAT in a mouse model for DIO. Our findings have laid out the foundation for future clinical studies using optoacoustic-derived vascular readouts from adipose tissues as a biomarker for monitoring microcirculatory function in metabolic disease.

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对接受运动干预的饮食诱导型肥胖小鼠真皮微循环血管再造进行无创光声成像

在肥胖者的真皮白色脂肪组织（dWAT）和皮下白色脂肪组织（scWAT）中已观察到微循环功能障碍，并被认为是心血管代谢疾病进展的早期预测标志物。对这些组织中的微血管重塑进行体内可视化和纵向监测仍具有挑战性。我们比较了两种光声成像方法的性能，即多光谱光声断层扫描（MSOT）和光栅扫描光声介孔镜（RSOM），这两种方法都能在小鼠自愿轮跑干预 32 周的节食诱导肥胖（DIO）模型中观察到 scWAT 和 dWAT 中的脂质和血红蛋白对比度。即使在 DIO 的早期阶段，MSOT 也能定量地观察到小鼠脂肪库中的脂质和血红蛋白对比。据我们所知，我们首次发现 RSOM 可以精确观察 dWAT 的微血管，并提供 dWAT 和真皮层的皮层厚度和血管密度的定量读数。结合使用 MSOT 和 RSOM 可以解决运动引起的 dWAT 和 scWAT 微血管密度、组织氧饱和度、脂质和血容量的形态学变化。结合使用 MSOT 和 RSOM 可精确监测 DIO 小鼠模型中 dWAT 和 scWAT 的微循环功能障碍和干预反应。我们的研究结果为今后利用脂肪组织的光声血管读数作为生物标记监测代谢性疾病微循环功能的临床研究奠定了基础。

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来源期刊

Photoacoustics Physics and Astronomy-Atomic and Molecular Physics, and Optics

CiteScore

11.40

自引率

16.50%

发文量

审稿时长

53 days

期刊介绍： The open access Photoacoustics journal (PACS) aims to publish original research and review contributions in the field of photoacoustics-optoacoustics-thermoacoustics. This field utilizes acoustical and ultrasonic phenomena excited by electromagnetic radiation for the detection, visualization, and characterization of various materials and biological tissues, including living organisms. Recent advancements in laser technologies, ultrasound detection approaches, inverse theory, and fast reconstruction algorithms have greatly supported the rapid progress in this field. The unique contrast provided by molecular absorption in photoacoustic-optoacoustic-thermoacoustic methods has allowed for addressing unmet biological and medical needs such as pre-clinical research, clinical imaging of vasculature, tissue and disease physiology, drug efficacy, surgery guidance, and therapy monitoring. Applications of this field encompass a wide range of medical imaging and sensing applications, including cancer, vascular diseases, brain neurophysiology, ophthalmology, and diabetes. Moreover, photoacoustics-optoacoustics-thermoacoustics is a multidisciplinary field, with contributions from chemistry and nanotechnology, where novel materials such as biodegradable nanoparticles, organic dyes, targeted agents, theranostic probes, and genetically expressed markers are being actively developed. These advanced materials have significantly improved the signal-to-noise ratio and tissue contrast in photoacoustic methods.