The Open SprayBot: A high-throughput paper spray mass spectrometry platform for disease screening

IF 2 Q3 ENGINEERING, ELECTRICAL & ELECTRONIC

HardwareX Pub Date : 2024-06-30 DOI:10.1016/j.ohx.2024.e00551

Nancy Shyrley García-Rojas , Héctor Guillén-Alonso , Scott MacKay , Claudia Torres-Calzada , Leonardo Daniel Soto-Rodriguez , Robert Winkler , David S. Wishart

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引用次数: 0

Abstract

Newborn disease screening increases survival, improves quality of life and reduces treatment costs for healthcare systems. Mass spectrometry (MS) is an effective method for metabolic screening. However, conventional analytical methods require biofluid handling and cooling conditions during transport, making the logistics difficult and expensive, especially for remote regions. ’Paper-spray’ (PS) ionization generates a charged solvent spray from samples deposited on paper strips. Therefore, samples can be applied on a suitable matrix and shipped as dried spots to diagnostic laboratories with standard postal or messenger services. We built a robotic platform, the ’Open SprayBot’, to automatically analyze paper-deposited samples via PS-MS and increase the sample throughput. The system is operated via RUMBA32 and Arduino Mega boards. A commercial syringe pump and power supply provide solvent application and electrical current required for PS-MS. The usability of the Open SprayBot was demonstrated by quantifying palmitoyl-l-carnitine, a common biomarker in newborn screening.

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开放喷雾机器人：用于疾病筛查的高通量纸喷雾质谱平台

新生儿疾病筛查可提高存活率、改善生活质量并降低医疗系统的治疗成本。质谱法（MS）是一种有效的代谢筛查方法。然而，传统的分析方法需要生物流体处理和运输过程中的冷却条件，因此物流困难且成本高昂，尤其是对于偏远地区而言。纸喷雾"（PS）离子化技术可使沉积在纸条上的样品产生带电溶剂喷雾。因此，可以将样品涂抹在合适的基质上，然后通过标准的邮政或信使服务，以干燥点的形式运送到诊断实验室。我们建立了一个机器人平台--"Open SprayBot"，通过 PS-MS 自动分析沉积在纸上的样品，并提高样品吞吐量。该系统通过 RUMBA32 和 Arduino Mega 板进行操作。商用注射泵和电源提供 PS-MS 所需的溶剂应用和电流。Open SprayBot 的实用性通过对棕榈酰左旋肉碱（新生儿筛查中的一种常见生物标志物）的定量进行了验证。

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来源期刊

HardwareX Engineering-Industrial and Manufacturing Engineering

CiteScore

4.10

自引率

18.20%

发文量

124

审稿时长

24 weeks

期刊介绍： HardwareX is an open access journal established to promote free and open source designing, building and customizing of scientific infrastructure (hardware). HardwareX aims to recognize researchers for the time and effort in developing scientific infrastructure while providing end-users with sufficient information to replicate and validate the advances presented. HardwareX is open to input from all scientific, technological and medical disciplines. Scientific infrastructure will be interpreted in the broadest sense. Including hardware modifications to existing infrastructure, sensors and tools that perform measurements and other functions outside of the traditional lab setting (such as wearables, air/water quality sensors, and low cost alternatives to existing tools), and the creation of wholly new tools for either standard or novel laboratory tasks. Authors are encouraged to submit hardware developments that address all aspects of science, not only the final measurement, for example, enhancements in sample preparation and handling, user safety, and quality control. The use of distributed digital manufacturing strategies (e.g. 3-D printing) is encouraged. All designs must be submitted under an open hardware license.