CD4+ T-cell subsets are associated with chronic stress effects in newly diagnosed anxiety disorders

IF 4.3 2区 医学 Q1 NEUROSCIENCES
Bindong Dai, Tao Li, Jinya Cao, Xiaohui Zhao, Yinan Jiang, Lili Shi, Jing Wei
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引用次数: 0

Abstract

Aim

Prior research has indicated a connection between CD4+ T cells and the development of anxiety, but the specific CD4+ T cell subsets linked to anxiety disorders remain uncertain. Our study seeks to investigate the relationship between distinct CD4+ T cell subsets and anxiety, as well as to explore whether CD4+ T cell subsets mediate the effect of chronic psychological stress on anxiety.

Methods

56 eligible matched participants were recruited in Peking Union Medical College Hospital. The diagnosis was made based on DSM-5 diagnostic criteria. The severity of anxiety and depression symptoms was assessed using the Hamilton Anxiety Rating Scale and Hamilton Depression Rating Scale, respectively. The Life Events Scale (LES) evaluated the chronic stress level. CD4+ T cell subsets were characterized using multiparametric flow cytometry. To assess the impact of CD4+ T cells on the effect of chronic psychological stress on anxiety, Partial Least Squares Structural Equation Modeling (PLS-SEM) analysis was employed.

Results

We discovered fifteen notably distinct CD4+ T-cell subsets in anxiety disorder patients compared to healthy controls. Multiple linear regression analysis unveiled an association between anxiety severity and CD27+CD45RA Th cells, CD27+CD28+ Tregs, and the total Life Events Scale (LES) score. The PLS-SEM analysis demonstrated that CD4+ T cell subsets and LES could explain 80.2% of the variance in anxiety. Furthermore, it was observed that CD27+CD28+ Th/Treg cells acted as inverse mediators of the effects of LES on anxiety (P = 0.031).

Conclusions

Drug naïve anxiety disorder patients exhibited significant alterations in numerous CD4+ T-cell subsets. Specifically, the memory subset of CD27+CD45RA Th cells and the naïve subset of CD27+CD28+ Treg cells were found to be independent factors associated with the severity of anxiety. Additionally, the CD27+CD28+ Th and Treg cell subsets played a significant mediating role in the influence of long-term psychological stress on anxiety.

CD4+ T 细胞亚群与新诊断焦虑症的慢性压力效应有关
目的已有研究表明 CD4+ T 细胞与焦虑症的发生有关,但与焦虑症有关的特定 CD4+ T 细胞亚群仍不确定。我们的研究旨在探讨不同的 CD4+ T 细胞亚群与焦虑之间的关系,并探讨 CD4+ T 细胞亚群是否介导了慢性心理压力对焦虑的影响。根据 DSM-5 诊断标准进行诊断。焦虑和抑郁症状的严重程度分别使用汉密尔顿焦虑评定量表和汉密尔顿抑郁评定量表进行评估。生活事件量表(LES)评估了慢性压力水平。CD4+ T细胞亚群采用多参数流式细胞术进行鉴定。为了评估 CD4+ T 细胞对慢性心理压力对焦虑的影响,我们采用了偏最小二乘法结构方程建模(PLS-SEM)分析。多元线性回归分析揭示了焦虑症严重程度与 CD27+CD45RA- Th 细胞、CD27+CD28+ Tregs 和生活事件量表(LES)总分之间的关系。PLS-SEM分析表明,CD4+ T细胞亚群和LES可解释80.2%的焦虑变异。此外,还观察到 CD27+CD28+ Th/Treg 细胞是 LES 对焦虑影响的反向中介(P = 0.031)。具体而言,CD27+CD45RA-Th 细胞记忆亚群和 CD27+CD28+ Treg 细胞幼稚亚群是与焦虑严重程度相关的独立因素。此外,CD27+CD28+ Th 和 Treg 细胞亚群在长期心理压力对焦虑的影响中起着重要的中介作用。
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来源期刊
Neurobiology of Stress
Neurobiology of Stress Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
9.40
自引率
4.00%
发文量
74
审稿时长
48 days
期刊介绍: Neurobiology of Stress is a multidisciplinary journal for the publication of original research and review articles on basic, translational and clinical research into stress and related disorders. It will focus on the impact of stress on the brain from cellular to behavioral functions and stress-related neuropsychiatric disorders (such as depression, trauma and anxiety). The translation of basic research findings into real-world applications will be a key aim of the journal. Basic, translational and clinical research on the following topics as they relate to stress will be covered: Molecular substrates and cell signaling, Genetics and epigenetics, Stress circuitry, Structural and physiological plasticity, Developmental Aspects, Laboratory models of stress, Neuroinflammation and pathology, Memory and Cognition, Motivational Processes, Fear and Anxiety, Stress-related neuropsychiatric disorders (including depression, PTSD, substance abuse), Neuropsychopharmacology.
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