Splenocytes and thymocytes migration patterns between lymphoid organs in pregnancy

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2024-07-02 DOI:10.1016/j.bbrep.2024.101769

Gabriela T. Cruz-Cureño , Marina Ch Rosales-Tarteaut , Lourdes A. Arriaga-Pizano , Luvia E. Sánchez-Torres , Denisse Castro-Eguiluz , Jessica L. Prieto-Chávez , Rodolfo Pastelin-Palacios , Ana Flisser , Arturo Cérbulo-Vázquez

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引用次数: 0

Abstract

Background

Cell migration is essential for the immune system and is frequently analyzed in adult non-pregnant animals but poorly explored in pregnant animals. However, a physiologic increased size in the spleen and periaortic lymph nodes had been reported in pregnant mice.

Methods

Using a mouse model, we transferred PKH26-stained thymocytes and splenocytes from pregnant or non-pregnant animals to receptor mice in the presence or absence of pregnancy. Percentage of PKH-26 cells and Mean Fluorescence Intensity were calculated. Non-parametric ANOVA analysis was performed.

Results

We detected that the percentage of PKH26+ thymocytes in the spleen, lymph nodes, and peripheral blood is higher in females than in males (p = 0.039). Our results showed a similar frequency of thymocytes and splenocytes from pregnant and non-pregnant mice located in receptor lymphoid organs (p > 0.05). Also, the location of marked cells was similar during the perinatal period (p > 0.05).

Conclusions

The mobility of thymocytes and splenocytes in pregnant and non-pregnant mice is similar. Therefore, we suggest that the larger size of the spleen and periaortic lymph nodes noted previously in pregnant mice could result from the retention of leukocytes in the secondary lymphoid organs.

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妊娠期脾细胞和胸腺细胞在淋巴器官之间的迁移模式

背景细胞迁移对免疫系统至关重要，经常对成年非妊娠动物进行分析，但对妊娠动物的研究却很少。方法利用小鼠模型，我们将妊娠或未妊娠动物的 PKH26 染色胸腺细胞和脾细胞转移到受体小鼠体内。计算 PKH-26 细胞的百分比和平均荧光强度。结果我们发现，雌性小鼠脾脏、淋巴结和外周血中PKH26+胸腺细胞的百分比高于雄性小鼠（P = 0.039）。我们的结果显示，妊娠小鼠和非妊娠小鼠的胸腺细胞和脾脏细胞位于受体淋巴器官的频率相似（p >0.05）。结论怀孕小鼠和非怀孕小鼠胸腺细胞和脾脏细胞的流动性相似。因此，我们认为，以前注意到的妊娠小鼠脾脏和主动脉周围淋巴结较大的现象可能是由于白细胞滞留在次级淋巴器官中造成的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.