{"title":"In-silico studies of isolated biological active fractions of Vachellia nilotica leaves extract against MG 63 cell lines and biological applications","authors":"Neha Verma , Mohini Singh, Shilpa Kumari, Kanu Priya","doi":"10.1016/j.bcab.2024.103276","DOIUrl":null,"url":null,"abstract":"<div><p>Osteosarcoma weakens the bone by destroying tissue. It originates from immature bone cells, which typically give rise to new bone tissue. Medicinal herbs are a potential source for an extensive range of phytochemicals with anticipated anticancer activity. The present study was to form an impression of the medicinal herb <em>Vachellia nilotica</em> leaves extract and its fractions as a cancer therapeutic agent against Osteosarcoma cell lines (MG 63). This study used the MTT assay, DPPH, Cell cycle analysis, and Alizarin red S dye to investigate the effects of crude ethyl acetate extract and its fractions on viability, antioxidant capacity, and mineralization, respectively. To determine the pharmacokinetic properties of the discovered substances and assess their potential toxicity, computer-assisted analysis was performed.</p><p>The investigation reveals that fraction 1E considerably lowers the percentage viability of human osteosarcoma MG 63. The effect was shown in the dose and time-dependent manner. Furthermore, fraction 1E exhibited the highest radical scavenging activity and showed the least mineralizing activity after the treatment. Positive pharmacokinetic properties of the phytochemicals were observed, making them an excellent starting point for the development of drugs. Thus, the present study highlighted the potency of the <em>V.nilotica</em> ethyl acetate extract and its bioactive fractions against Osteosarcoma and their role in the cell cycle. The study also concludes the <em>In-silico</em> targeted proteins about the disease, which is the first time reported as per our knowledge and the literature available.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biocatalysis and agricultural biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1878818124002603","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Osteosarcoma weakens the bone by destroying tissue. It originates from immature bone cells, which typically give rise to new bone tissue. Medicinal herbs are a potential source for an extensive range of phytochemicals with anticipated anticancer activity. The present study was to form an impression of the medicinal herb Vachellia nilotica leaves extract and its fractions as a cancer therapeutic agent against Osteosarcoma cell lines (MG 63). This study used the MTT assay, DPPH, Cell cycle analysis, and Alizarin red S dye to investigate the effects of crude ethyl acetate extract and its fractions on viability, antioxidant capacity, and mineralization, respectively. To determine the pharmacokinetic properties of the discovered substances and assess their potential toxicity, computer-assisted analysis was performed.
The investigation reveals that fraction 1E considerably lowers the percentage viability of human osteosarcoma MG 63. The effect was shown in the dose and time-dependent manner. Furthermore, fraction 1E exhibited the highest radical scavenging activity and showed the least mineralizing activity after the treatment. Positive pharmacokinetic properties of the phytochemicals were observed, making them an excellent starting point for the development of drugs. Thus, the present study highlighted the potency of the V.nilotica ethyl acetate extract and its bioactive fractions against Osteosarcoma and their role in the cell cycle. The study also concludes the In-silico targeted proteins about the disease, which is the first time reported as per our knowledge and the literature available.
骨肉瘤通过破坏组织来削弱骨骼。它起源于未成熟的骨细胞，这些细胞通常会产生新的骨组织。药用草本植物是多种具有预期抗癌活性的植物化学物质的潜在来源。本研究旨在了解药用植物黑叶穗醋栗叶提取物及其馏分对骨肉瘤细胞系（MG 63）的癌症治疗作用。本研究使用 MTT 法、DPPH 法、细胞周期分析法和茜素红 S 染料分别研究了乙酸乙酯粗提取物及其馏分对细胞活力、抗氧化能力和矿化作用的影响。为了确定所发现物质的药代动力学特性并评估其潜在毒性，研究人员进行了计算机辅助分析。研究发现，馏分 1E 能显著降低人骨肉瘤 MG 63 的存活率，其影响呈剂量和时间依赖性。此外，馏分 1E 在治疗后表现出最高的自由基清除活性和最低的矿化活性。研究还观察到这些植物化学物质具有积极的药代动力学特性，这使它们成为开发药物的绝佳起点。因此，本研究强调了尼罗河流域植物乙酸乙酯提取物及其生物活性馏分对骨肉瘤的功效及其在细胞周期中的作用。本研究还总结出了有关该疾病的硅内靶向蛋白质，这是根据我们的知识和现有文献首次报道。
期刊介绍：
Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.
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