Bioactivities evaluation of an endophytic bacterial strain Bacillus tequilensis QNF2 inhibiting apple ring rot caused by Botryosphaeria dothidea on postharvest apple fruits

IF 4.5 1区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yonghong Huang , Jinghui Li , Xiaoying Shan , Hanting Wang , Yanxin Duan
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引用次数: 0

Abstract

Apple ring rot, one of the most common apple postharvest diseases during storage, is caused by Botryosphaeria dothidea. Presently, the disease management is primarily dependent on chemical fungicide application. Here we demonstrated an endophyte bacterium Bacillus tequilensis QNF2, isolated from Chinese leek (Allium tuberosum) roots considerably suppressed B. dothidea mycelial growth, with the highest suppression of 73.56 % and 99.5 % in the PDA and PDB medium, respectively in vitro confront experiments. In in vivo experiments, B. tequilensis QNF2 exhibited a control efficacy of 88.52 % and 100 % on ring rot disease on postharvest apple fruits inoculated with B. dothidea disc and dipped into B. dothidea culture, respectively. In addition, B. tequilensis QNF2 volatile organic compounds (VOCs) also manifested markedly inhibition against B. dothidea mycelial growth and the ring rot on postharvest apple fruits. Moreover, B. tequilensis QNF2 severely damaged the mycelial morphology of B. dothidea. Finally, B. tequilensis QNF2 significantly repressed the expression of six pathogenicity-related genes, such as adh, aldh, aldh3, galm, pdc1, pdc2, involved in glycolysis/gluconeogenesis of B. dothidea. The findings of the study proved that B. tequilensis QNF2 was a promising alternative for controlling apple ring rot of postharvest apple fruit.

内生细菌菌株芽孢杆菌(Bacillus tequilensis QNF2)抑制采后苹果果实上由多蒂腐霉菌(Botryosphaeria dothidea)引起的苹果环腐病的生物活性评估
苹果环腐病是苹果贮藏过程中最常见的采后病害之一,由 Botryosphaeria dothidea 引起。目前,病害的防治主要依赖于化学杀菌剂的施用。在此,我们证明了一种从中国韭菜(Allium tuberosum)根中分离出来的内生细菌 Bacillus tequilensis QNF2 能显著抑制 B. dothidea 菌丝的生长,在 PDA 和 PDB 培养基的体外对抗实验中,最高抑制率分别为 73.56 % 和 99.5 %。在体内实验中,B. tequilensis QNF2 对接种了 B. dothidea 菌盘和浸入 B. dothidea 培养液的采后苹果果实上的环腐病的防治效果分别为 88.52 % 和 100 %。此外,B. tequilensis QNF2 挥发性有机化合物(VOCs)也对多蒂德氏菌菌丝生长和采后苹果果实上的环腐病有明显的抑制作用。此外,B. tequilensis QNF2 还严重破坏了 B. dothidea 的菌丝形态。最后,B. tequilensis QNF2 显著抑制了 6 个与致病性相关的基因的表达,如 adh、aldh、aldh3、galm、pdc1、pdc2,这些基因参与了 B. dothidea 的糖酵解/葡萄糖生成。研究结果证明,B. tequilensis QNF2 是控制采后苹果果实苹果环腐病的一种有前途的替代方法。
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来源期刊
Food microbiology
Food microbiology 工程技术-生物工程与应用微生物
CiteScore
11.30
自引率
3.80%
发文量
179
审稿时长
44 days
期刊介绍: Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.
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