Comparative genomics of canine parvovirus in South America: Diversification patterns in local populations

IF 2.6 4区医学 Q3 INFECTIOUS DISEASES

Infection Genetics and Evolution Pub Date : 2024-07-03 DOI:10.1016/j.meegid.2024.105633

Sofía Grecco , Emma Condon , Danilo Bucafusco , Ana Cristina Bratanich , Yanina Panzera , Ruben Pérez

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引用次数: 0

Abstract

Canine parvovirus (CPV) is a significant pathogen in domestic dogs worldwide, causing a severe and often fatal disease. CPV comprises three antigenic variants (2a, 2b, and 2c) distributed unevenly among several phylogenetic groups. The present study compared genetic variability and evolutionary patterns in South American CPV populations. We collected samples from puppies suspected of CPV infection in the neighboring Argentina and Uruguay. Antigenic variants were preliminarily characterized using PCR-RFLP and partial vp2 sequencing. Samples collected in Argentina during 2008–2018 were mainly of the 2c variant. In the Uruguayan strains (2012–2019), the 2a variant wholly replaced the 2c from 2014. Full-length coding genome and vp2 sequences were compared with global strains. The 2c and 2a strains fell by phylogenetic analysis into two phylogroups (Europe I and Asia I). The 2c strains from Argentina and Uruguay clustered in the Europe I group, with strains from America, Europe, Asia, and Oceania. Europe I is widely distributed in South America in the dog population and is also being detected in the wildlife population. The 2a strains from Uruguay formed the distinct Asia I group with strains from Asia, Africa, America, and Oceania. This Asia I group is increasing its distribution in South America and worldwide. Our research reveals high genetic variability in adjacent synchronic samples and different evolutionary patterns in South American CPV. We also highlight the importance of ancestral migrations and local diversification in the evolution of global CPV strains.

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南美洲犬细小病毒的比较基因组学：当地种群的多样化模式。

犬细小病毒（CPV）是全球家犬的一种重要病原体，可导致严重的疾病，而且往往是致命的。CPV 包括三种抗原变体（2a、2b 和 2c），在几个系统发育群中分布不均。本研究比较了南美洲 CPV 群体的遗传变异和进化模式。我们从邻近的阿根廷和乌拉圭收集了疑似感染 CPV 的幼犬样本。利用 PCR-RFLP 和部分 vp2 测序初步确定了抗原变异的特征。2008-2018 年期间在阿根廷采集的样本主要是 2c 变异株。在乌拉圭的菌株中（2012-2019 年），2a 变种从 2014 年起完全取代了 2c。全长编码基因组和 vp2 序列与全球菌株进行了比较。通过系统发育分析，2c 和 2a 菌株分为两个系统群（欧洲 I 和亚洲 I）。来自阿根廷和乌拉圭的 2c 菌株与来自美洲、欧洲、亚洲和大洋洲的菌株归入欧洲 I 组。欧洲 I 型广泛分布于南美洲的狗群中，在野生动物群中也有发现。乌拉圭的 2a 株系与来自亚洲、非洲、美洲和大洋洲的株系组成了独特的亚洲 I 组。这个亚洲 I 组在南美洲和全球的分布日益扩大。我们的研究揭示了南美 CPV 相邻同步样本的高遗传变异性和不同的进化模式。我们还强调了祖先迁徙和局部多样化在全球 CPV 株系进化中的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Infection Genetics and Evolution 医学-传染病学

CiteScore

8.40

自引率

0.00%

发文量

215

审稿时长

82 days

期刊介绍： (aka Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases -- MEEGID) Infectious diseases constitute one of the main challenges to medical science in the coming century. The impressive development of molecular megatechnologies and of bioinformatics have greatly increased our knowledge of the evolution, transmission and pathogenicity of infectious diseases. Research has shown that host susceptibility to many infectious diseases has a genetic basis. Furthermore, much is now known on the molecular epidemiology, evolution and virulence of pathogenic agents, as well as their resistance to drugs, vaccines, and antibiotics. Equally, research on the genetics of disease vectors has greatly improved our understanding of their systematics, has increased our capacity to identify target populations for control or intervention, and has provided detailed information on the mechanisms of insecticide resistance. However, the genetics and evolutionary biology of hosts, pathogens and vectors have tended to develop as three separate fields of research. This artificial compartmentalisation is of concern due to our growing appreciation of the strong co-evolutionary interactions among hosts, pathogens and vectors. Infection, Genetics and Evolution and its companion congress [MEEGID](http://www.meegidconference.com/) (for Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases) are the main forum acting for the cross-fertilization between evolutionary science and biomedical research on infectious diseases. Infection, Genetics and Evolution is the only journal that welcomes articles dealing with the genetics and evolutionary biology of hosts, pathogens and vectors, and coevolution processes among them in relation to infection and disease manifestation. All infectious models enter the scope of the journal, including pathogens of humans, animals and plants, either parasites, fungi, bacteria, viruses or prions. The journal welcomes articles dealing with genetics, population genetics, genomics, postgenomics, gene expression, evolutionary biology, population dynamics, mathematical modeling and bioinformatics. We also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services .