Genetic variability of human papillomavirus type 18 based on E6, E7 and L1 genes in central China.

IF 4 3区医学 Q2 VIROLOGY

Virology Journal Pub Date : 2024-07-05 DOI:10.1186/s12985-024-02424-9

Ting Li, Zhiping Yang, Ping Luo, Yang Yang, Zicong Lin, Bing Mei

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引用次数: 0

Abstract

Background: High-risk human papillomavirus (HR-HPV) infection is an important factor for the development of cervical cancer. HPV18 is the second most common HR-HPV after HPV16.

Methods: In this study, MEGA11 software was used to analyze the variation and phylogenetic tree of HPV18 E6-E7 and L1 genes. The selective pressure to E6, E7 and L1 genes was estimated using pamlX. In addition, the B cell epitopes of L1 amino acid sequences and T cell epitopes of E6-E7 amino acid sequences in HPV18 were predicted by ABCpred server and IEDB website, respectively.

Results: A total of 9 single nucleotide variants were found in E6-E7 sequences, of which 2 were nonsynonymous variants and 7 were synonymous variants. Twenty single nucleotide variants were identified in L1 sequence, including 11 nonsynonymous variants and 9 synonymous variants. Phylogenetic analysis showed that E6-E7 and L1 sequences were all distributed in A lineage. In HPV18 E6, E7 and L1 sequences, no positively selected site was found. The nonconservative substitution R545C in L1 affected hypothetical B cell epitope. Two nonconservative substitutions, S82A in E6, and R53Q in E7, impacted multiple hypothetical T cell epitopes.

Conclusion: The sequence variation data of HPV18 may lay a foundation for the virus diagnosis, further study of cervical cancer and vaccine design in central China.

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华中地区基于 E6、E7 和 L1 基因的人类乳头瘤病毒 18 型遗传变异。

背景：高危人乳头瘤病毒（HR-HPV）感染是宫颈癌发病的一个重要因素。HPV18是继HPV16之后第二种最常见的HR-HPV：本研究使用 MEGA11 软件分析了 HPV18 E6-E7 和 L1 基因的变异和系统发生树。使用 pamlX 估算了 E6、E7 和 L1 基因的选择压力。此外，还利用 ABCpred 服务器和 IEDB 网站分别预测了 HPV18 中 L1 氨基酸序列的 B 细胞表位和 E6-E7 氨基酸序列的 T 细胞表位：结果：E6-E7序列中共发现9个单核苷酸变异，其中2个为非同义变异，7个为同义变异。在 L1 序列中发现了 20 个单核苷酸变异，包括 11 个非同义变异和 9 个同义变异。系统发育分析表明，E6-E7和L1序列均分布在A系中。在 HPV18 E6、E7 和 L1 序列中，没有发现正选择位点。L1 中的非保守替换 R545C 影响了假定的 B 细胞表位。E6 中的 S82A 和 E7 中的 R53Q 这两个非保守替换影响了多个假定的 T 细胞表位：HPV18的序列变异数据可为华中地区的病毒诊断、宫颈癌的进一步研究和疫苗设计奠定基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Virology Journal 医学-病毒学

CiteScore

7.40

自引率

2.10%

发文量

186

审稿时长

1 months

期刊介绍： Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies. The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.