Chronic exposure to TiO2 micro- and nano particles: A biochemical and histopathological experimental study

IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL
Mariela Gisele Domingo, Melisa Kurtz, Guillermo Maglione, Maximiliano Martin, Fernando Brites, Deborah Ruth Tasat, Daniel Gustavo Olmedo
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引用次数: 0

Abstract

The aim of this work was to analyze the effects of long-term exposure to titanium dioxide (TiO2) micro- (MPs) and nanoparticles (NPs) (six and 12 months) on the biochemical and histopathological response of target organs using a murine model. Male Wistar rats were intraperitoneally injected with a suspension of TiO2 NPs (5 nm; TiO2-NP5 group) or MPs (45 μm; TiO2-NP5 group); the control group was injected with saline solution. Six and 12 months post-injection, titanium (Ti) concentration in plasma and target organs was determined spectrometrically (ICP-MS). Blood smears and organ tissue samples were evaluated by light microscopy. Liver and kidney function was evaluated using serum biochemical parameters. Oxidative metabolism was assessed 6 months post-injection (determination of superoxide anion by nitroblue tetrazolium (NBT) test, superoxide dismutase (SOD) and catalase (CAT), lipid peroxidation, and paraoxonase 1). Titanium (Ti) concentration in target organs and plasma was significantly higher in the TiO2-exposed groups than in the control group. Histological evaluation showed the presence of titanium-based particles in the target organs, which displayed no structural alterations, and in blood monocytes. Oxidative metabolism analysis showed that TiO2 NPs were more reactive over time than MPs (p < .05) and mobilization of antioxidant enzymes and membrane damage varied among the studied organs. Clearance of TiO2 micro and nanoparticles differed among the target organs, and lung clearance was more rapid than clearance from the lungs and kidneys (p < .05). Conversely, Ti concentration in plasma increased with time (p < .05). In conclusion, neither serum biochemical parameters nor oxidative metabolism markers appear to be useful as biomarkers of tissue damage in response to TiO2 micro- and nanoparticle deposits at chronic time points.

长期接触二氧化钛微粒和纳米微粒:生化和组织病理学实验研究
本研究的目的是利用小鼠模型分析长期暴露于二氧化钛(TiO2)微颗粒(MPs)和纳米颗粒(NPs)(6 个月和 12 个月)对靶器官生化和组织病理学反应的影响。雄性 Wistar 大鼠腹腔注射 TiO2 NPs(5 纳米;TiO2-NP5 组)或 MPs(45 微米;TiO2-NP5 组)悬浮液;对照组注射生理盐水。注射后 6 个月和 12 个月,血浆和靶器官中的钛(Ti)浓度用光谱法(ICP-MS)测定。用光学显微镜对血液涂片和器官组织样本进行评估。利用血清生化指标对肝肾功能进行评估。对注射后 6 个月的氧化代谢进行了评估(通过硝基蓝四氮唑(NBT)测试测定超氧阴离子、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)、脂质过氧化和副氧合酶 1)。目标器官和血浆中的钛(Ti)浓度在二氧化钛暴露组明显高于对照组。组织学评估显示,靶器官和血液单核细胞中存在钛基微粒,但未出现结构性改变。氧化代谢分析表明,随着时间的推移,TiO2 NPs 比 MPs 更具活性(p <.05),抗氧化酶的调动和膜损伤在所研究的器官中各不相同。各目标器官对二氧化钛微粒和纳米粒子的清除率不同,肺部的清除率比肺部和肾脏的清除率更快(p <.05)。相反,血浆中的钛浓度随着时间的推移而增加(p < .05)。总之,无论是血清生化指标还是氧化代谢标志物,似乎都不能作为组织在长期时间点上受到二氧化钛微粒和纳米粒子沉积物损伤的生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.50
自引率
2.90%
发文量
199
审稿时长
12 months
期刊介绍: Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.
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