Prognostic Value of Cancer-Associated Fibroblast Marker Expression in the Intratumoral and Marginal Areas of Soft Tissue Sarcoma.

IF 3.5 4区医学 Q3 CELL BIOLOGY

Pathobiology Pub Date : 2024-07-22 DOI:10.1159/000539855

Michinobu Umakoshi, Yukitsugu Kudo-Asabe, Hiroyuki Tsuchie, Zhuo Li, Kei Koyama, Ken Miyabe, Makoto Yoshida, Hiroyuki Nagasawa, Hiroshi Nanjo, Kyoji Okada, Daichi Maeda, Naohisa Miyakoshi, Masamitsu Tanaka, Akiteru Goto

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引用次数: 0

Abstract

Introduction: The tumor microenvironment of sarcomas has not been studied in detail; in particular, little is known about cancer-associated fibroblasts (CAFs). Sarcoma cells are difficult to distinguish from CAFs, either histomorphologically or immunohistochemically.

Methods: We scored the expression of individual CAF markers (fibroblast-activating protein [FAP], CD10, and podoplanin) in the intratumoral and marginal areas of 133 sarcomas. We also examined the association between these markers, as well as the number of CD163-positive macrophages (i.e., tumor-associated macrophages), and clinical outcome.

Results: In all cases, the log-rank test revealed that those with high marker scores and macrophage counts (except for marginal CD10+ CAFs) showed significantly worse disease-free survival (DFS). Grade 2/3 cases with high CAF scores (excluding the marginal FAP and CD10 scores) showed significantly worse DFS, whereas those with high intratumoral FAP/CD10 and marginal podoplanin scores showed significantly worse metastasis-free survival (MFS), and those with high intratumoral CD10 score showed significantly worse local recurrence-free survival (LFS). Multivariate analysis identified intratumoral CD10/podoplanin scores and marginal FAP/podoplanin scores as independent prognostic factors for DFS, intratumoral FAP/CD10 and marginal FAP/podoplanin/CD163-positive macrophage scores as independent prognostic factors for MFS, and the intratumoral podoplanin score as an independent prognostic factor for LFS. There was a weak-to-moderate correlation between each score and CD163-positive macrophage counts.

Conclusion: Patients with high CAF marker expression in the intratumoral and marginal areas have a poorer outcome.

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软组织肉瘤瘤内和边缘区域 CAF 标记表达的预后价值。

简介肉瘤的肿瘤微环境尚未得到详细研究，尤其是对癌症相关成纤维细胞（CAFs）知之甚少。肉瘤细胞与成纤维细胞很难从组织形态学或免疫组化学上区分开来：我们对 133 例肉瘤瘤内和边缘区域的 CAF 标记（成纤维细胞活化蛋白（FAP）、CD10 和 podoplanin）的表达进行了评分。我们还研究了这些标记物以及 CD163 阳性巨噬细胞（即肿瘤相关巨噬细胞）的数量与临床结果之间的关系：对数秩检验显示，在所有病例中，标记物评分和巨噬细胞数量较高者（边缘CD10+ CAF除外）的无病生存期（DFS）明显较差。CAF评分（不包括边缘FAP和CD10评分）高的2/3级病例无病生存期（DFS）明显更差，而瘤内FAP/CD10和边缘荚膜评分高的病例无转移生存期（MFS）明显更差，瘤内CD10评分高的病例无局部复发生存期（LFS）明显更差。多变量分析确定瘤内CD10/podoplanin评分和边缘FAP/podoplanin评分是DFS的独立预后因素，瘤内FAP/CD10和边缘FAP/podoplanin/CD163阳性巨噬细胞评分是MFS的独立预后因素，瘤内podoplanin评分是LFS的独立预后因素。每个评分与CD163阳性巨噬细胞计数之间存在弱到中等程度的相关性：结论：瘤内和边缘区域CAF标记物表达较高的患者预后较差。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pathobiology 医学-病理学

CiteScore

8.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： ''Pathobiology'' offers a valuable platform for the publication of high-quality original research into the mechanisms underlying human disease. Aiming to serve as a bridge between basic biomedical research and clinical medicine, the journal welcomes articles from scientific areas such as pathology, oncology, anatomy, virology, internal medicine, surgery, cell and molecular biology, and immunology. Published bimonthly, ''Pathobiology'' features original research papers and reviews on translational research. The journal offers the possibility to publish proceedings of meetings dedicated to one particular topic.