Elastin-derived peptides (EDPs) as a potential pro-malignancy factor in human leukemia cell lines.

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
ACS Applied Electronic Materials Pub Date : 2024-10-01 Epub Date: 2024-07-05 DOI:10.1007/s12026-024-09511-7
Konrad A Szychowski, Bartosz Skóra
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Abstract

The extracellular matrix (ECM) is currently considered to be an important factor influencing the migration and progression of cancer cells. Therefore, the aim of our study was to investigate the mechanism of action of elastin-derived peptides in cancerous cells derived from the immunological system, i.e., HL-60, K562, and MEG-A2 cell lines. Moreover, an attempt to clarify the involvement of c-SRC kinase in EDP mechanism of action was also undertaken. Our data show that the VGVAPG and VVGPGA peptides are not toxic in the studied cell lines. Moreover, due to the involvement of KI67 and PCNA proteins in the cell cycle and proliferation, we can assume that neither peptide stimulates cell proliferation. Our data suggest that both peptides could initiate the differentiation process in all the studied cell lines. However, due to the different origins (HL-60 and K562-leukemic cell line vs. MEG-A2-megakaryoblastic origin) of the cell lines, the mechanism may differ. The increase in the ELANE mRNA expression noted in our experiments may also suggest enhancement of the migration of the tested cells. However, more research is needed to fully explain the mechanism of action of the VGVAPG and VVGPGA peptides in the HL-60, K562, and MEG-A2 cell lines. HIGHLIGHTS: • VGVAPG and VVGPGA peptides do not affect the metabolic activity of HL-60, K562, and MEG-A2 cells. • mTOR and PPARγ proteins are involved in the mechanism of action of VGVAPG and VVGPGA peptides. • Both peptides may initiate differentiation in HL-60, K562, and MEG-A2 cell lines.

Abstract Image

弹性蛋白衍生肽(EDPs)是人类白血病细胞系中潜在的促恶性因子。
细胞外基质(ECM)目前被认为是影响癌细胞迁移和发展的重要因素。因此,我们的研究旨在调查弹性蛋白衍生肽在来自免疫系统的癌细胞(即 HL-60、K562 和 MEG-A2 细胞系)中的作用机制。此外,我们还尝试阐明 c-SRC 激酶参与 EDP 作用机制的情况。我们的数据显示,VGVAPG 和 VVGPGA 肽对所研究的细胞系没有毒性。此外,由于 KI67 和 PCNA 蛋白参与细胞周期和增殖,我们可以认为这两种肽都不会刺激细胞增殖。我们的数据表明,这两种肽都能启动所有研究细胞系的分化过程。然而,由于细胞系的来源不同(HL-60 和 K562-白血病细胞系与 MEG-A2- 巨核细胞系),其机制可能也不同。在我们的实验中,ELANE mRNA 表达的增加也可能表明受试细胞的迁移能力增强。然而,要全面解释 VGVAPG 和 VVGPGA 肽在 HL-60、K562 和 MEG-A2 细胞系中的作用机制,还需要更多的研究。亮点:- VGVAPG 和 VVGPGA 肽不影响 HL-60、K562 和 MEG-A2 细胞的代谢活性。- mTOR 和 PPARγ 蛋白参与了 VGVAPG 和 VVGPGA 肽的作用机制。- 这两种肽都可以启动 HL-60、K562 和 MEG-A2 细胞系的分化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
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