{"title":"Application of RhD Blood Group to Simulate Antibody Identification Test in Immunohematology Education.","authors":"Yingying Niu, Jiang Wang, Yuxin Wang, Honghui Long, Danting Wang, Chunyan Huang","doi":"10.7754/Clin.Lab.2024.240225","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Immunohematology skill education is an important part of the transfusion medicine professional training. We tried to solve the difficulty of obtaining suitable and sufficient positive samples in the immunohematology education.</p><p><strong>Methods: </strong>Different identification panels and panel cells were created by RhD-positive red blood cells (RBCs) and RhD-negative RBCs, according to the underlying antibodies. Diluted anti-D reagent was used as simulated plasma for identification.</p><p><strong>Results: </strong>The antibody identification of single antibody with dose-effect and two antibodies present at the same time were successfully simulated.</p><p><strong>Conclusions: </strong>It is a practical and cheap method for antibody identification training to use RhD blood group, especially when positive samples are short.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"70 7","pages":""},"PeriodicalIF":0.7000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical laboratory","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.7754/Clin.Lab.2024.240225","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Immunohematology skill education is an important part of the transfusion medicine professional training. We tried to solve the difficulty of obtaining suitable and sufficient positive samples in the immunohematology education.
Methods: Different identification panels and panel cells were created by RhD-positive red blood cells (RBCs) and RhD-negative RBCs, according to the underlying antibodies. Diluted anti-D reagent was used as simulated plasma for identification.
Results: The antibody identification of single antibody with dose-effect and two antibodies present at the same time were successfully simulated.
Conclusions: It is a practical and cheap method for antibody identification training to use RhD blood group, especially when positive samples are short.
背景：免疫血液学技能教育是输血医学专业培训的重要组成部分。我们试图解决免疫血液学教学中难以获得合适且充足的阳性样本的问题：方法：根据RhD阳性红细胞（RBC）和RhD阴性红细胞（RBC）的基础抗体，建立不同的鉴定面板和面板细胞。用稀释的抗 D 试剂作为模拟血浆进行鉴定：结果：成功模拟了具有剂量效应的单抗体和同时存在两种抗体的抗体鉴定：结论：使用 RhD 血型进行抗体鉴定培训是一种实用、廉价的方法，尤其是在阳性样本较少的情况下。
期刊介绍：
Clinical Laboratory is an international fully peer-reviewed journal covering all aspects of laboratory medicine and transfusion medicine. In addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies. The journal publishes original articles, review articles, posters, short reports, case studies and letters to the editor dealing with 1) the scientific background, implementation and diagnostic significance of laboratory methods employed in hospitals, blood banks and physicians'' offices and with 2) scientific, administrative and clinical aspects of transfusion medicine and 3) in addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
