Quantitative Single-Cell Comparison of Sensitization to Radiation and a Radiomimetic Drug for Diverse Gold Nanoparticle Coatings

IF 11.1 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY
Douglas Howard, Tyron Turnbull, Puthenparampil Wilson, David John Paterson, Valentina Milanova, Benjamin Thierry, Ivan Kempson
{"title":"Quantitative Single-Cell Comparison of Sensitization to Radiation and a Radiomimetic Drug for Diverse Gold Nanoparticle Coatings","authors":"Douglas Howard, Tyron Turnbull, Puthenparampil Wilson, David John Paterson, Valentina Milanova, Benjamin Thierry, Ivan Kempson","doi":"10.1002/smsc.202400053","DOIUrl":null,"url":null,"abstract":"Metal-based nanoparticles (NPs) have entered clinical use for enhancing radiotherapy, but the underlying mechanisms remain ambiguous. Herein, single-cell analysis of two cell lines in response to megavolt irradiation and a radiomimetic drug, neocarzinostatin (NCS) after coculture with gold NPs with different surface coatings, polyethylene glycol (AuPEG), PEG, and transferrin (AuT) or silica (AuSiO<sub>2</sub>), is reported. Different surface chemistry presents a major challenge for objective comparison between the biological impacts where major differences in cell-uptake exist. AuSiO<sub>2</sub> NPs are the most efficient for promoting radiosensitization despite being associated with cells 10 times less than the actively targeted AuT NPs. Conversely, for cells exposed to NCS, AuSiO<sub>2</sub> NPs impede the radiomimetic action and promote cell survival. AuT NPs enhance death of cells in combination with NCS showing that NPs can sensitize against cytotoxic agents in addition to radiation. While NPs contribute to radiosensitization (or enhancing/impeding chemotherapeutic drug activity), due to cell and cell line heterogeneity, the ultimate radiosensitivity of a cell appears to be dominated by its inherent radiosensitivity and how this cell-regulated response is manipulated by NPs. This is evidenced through comparison of radiobiological response of cells with equivalent NP association rather than equivalent coculture conditions.","PeriodicalId":29791,"journal":{"name":"Small Science","volume":"25 1","pages":""},"PeriodicalIF":11.1000,"publicationDate":"2024-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Small Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/smsc.202400053","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

Abstract

Metal-based nanoparticles (NPs) have entered clinical use for enhancing radiotherapy, but the underlying mechanisms remain ambiguous. Herein, single-cell analysis of two cell lines in response to megavolt irradiation and a radiomimetic drug, neocarzinostatin (NCS) after coculture with gold NPs with different surface coatings, polyethylene glycol (AuPEG), PEG, and transferrin (AuT) or silica (AuSiO2), is reported. Different surface chemistry presents a major challenge for objective comparison between the biological impacts where major differences in cell-uptake exist. AuSiO2 NPs are the most efficient for promoting radiosensitization despite being associated with cells 10 times less than the actively targeted AuT NPs. Conversely, for cells exposed to NCS, AuSiO2 NPs impede the radiomimetic action and promote cell survival. AuT NPs enhance death of cells in combination with NCS showing that NPs can sensitize against cytotoxic agents in addition to radiation. While NPs contribute to radiosensitization (or enhancing/impeding chemotherapeutic drug activity), due to cell and cell line heterogeneity, the ultimate radiosensitivity of a cell appears to be dominated by its inherent radiosensitivity and how this cell-regulated response is manipulated by NPs. This is evidenced through comparison of radiobiological response of cells with equivalent NP association rather than equivalent coculture conditions.

Abstract Image

不同金纳米粒子涂层对辐射和仿辐射药物敏感性的单细胞定量比较
金属基纳米粒子(NPs)已进入临床应用,用于增强放射治疗,但其潜在机制仍不明确。本文报告了两种细胞系在与具有不同表面涂层(聚乙二醇(AuPEG)、PEG和转铁蛋白(AuT)或二氧化硅(AuSiO2))的金纳米粒子共培养后对兆伏特辐照和放射模拟药物新卡西诺司汀(NCS)的反应的单细胞分析。不同的表面化学成分对生物影响的客观比较提出了重大挑战,因为细胞吸收存在重大差异。尽管 AuSiO2 NPs 与细胞的关联度比主动靶向 AuT NPs 低 10 倍,但其促进放射增敏的效率最高。相反,对于暴露于 NCS 的细胞,AuSiO2 NPs 会阻碍辐射模拟作用并促进细胞存活。AuT NPs 在与 NCS 结合使用时会增强细胞的死亡,这表明 NPs 除辐射外还能对细胞毒性制剂产生增敏作用。虽然 NPs 有助于放射增敏(或增强/阻碍化疗药物活性),但由于细胞和细胞系的异质性,细胞的最终放射敏感性似乎受其固有的放射敏感性以及 NPs 如何操纵这种细胞调控反应的影响。这一点可以通过比较与等效 NP 结合而非等效共培养条件下细胞的放射生物学反应得到证明。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
14.00
自引率
2.40%
发文量
0
期刊介绍: Small Science is a premium multidisciplinary open access journal dedicated to publishing impactful research from all areas of nanoscience and nanotechnology. It features interdisciplinary original research and focused review articles on relevant topics. The journal covers design, characterization, mechanism, technology, and application of micro-/nanoscale structures and systems in various fields including physics, chemistry, materials science, engineering, environmental science, life science, biology, and medicine. It welcomes innovative interdisciplinary research and its readership includes professionals from academia and industry in fields such as chemistry, physics, materials science, biology, engineering, and environmental and analytical science. Small Science is indexed and abstracted in CAS, DOAJ, Clarivate Analytics, ProQuest Central, Publicly Available Content Database, Science Database, SCOPUS, and Web of Science.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信