A. M. Gordienko, L. A. Dadinova, M. V. Petoukhov, A. A. Mozhaev, V. A. Manuvera, V. N. Lazarev, E. V. Shtykova
{"title":"Oligomerization of the IHF Protein in the Presence of Metal Cations","authors":"A. M. Gordienko, L. A. Dadinova, M. V. Petoukhov, A. A. Mozhaev, V. A. Manuvera, V. N. Lazarev, E. V. Shtykova","doi":"10.1134/s1063774524600133","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The oligomeric state of the nucleoid-associated protein IHF (integration host factor) plays a significant role in the organization and compaction of the bacterial nucleoid and also in the evolution of bacterial resistance to unfavorable environmental conditions, in particular to antibiotics. Although IHF was identified more than 25 years ago, the molecular mechanisms of its participation in these processes are poorly understood. Using small-angle X-ray scattering, it was demonstrated for the first time that there are different oligomeric states of IHF in an aqueous medium depending on the presence of metal cations. It was found that the presence of Mg<sup>2+</sup> and K<sup>+</sup> ions hinders the formation of high-order oligomers of IHF. The results of this study may be useful in the development of strategies against bacterial resistance to drugs.</p>","PeriodicalId":527,"journal":{"name":"Crystallography Reports","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crystallography Reports","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1134/s1063774524600133","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CRYSTALLOGRAPHY","Score":null,"Total":0}
引用次数: 0
Abstract
The oligomeric state of the nucleoid-associated protein IHF (integration host factor) plays a significant role in the organization and compaction of the bacterial nucleoid and also in the evolution of bacterial resistance to unfavorable environmental conditions, in particular to antibiotics. Although IHF was identified more than 25 years ago, the molecular mechanisms of its participation in these processes are poorly understood. Using small-angle X-ray scattering, it was demonstrated for the first time that there are different oligomeric states of IHF in an aqueous medium depending on the presence of metal cations. It was found that the presence of Mg2+ and K+ ions hinders the formation of high-order oligomers of IHF. The results of this study may be useful in the development of strategies against bacterial resistance to drugs.
期刊介绍：
Crystallography Reports is a journal that publishes original articles short communications, and reviews on various aspects of crystallography: diffraction and scattering of X-rays, electrons, and neutrons, determination of crystal structure of inorganic and organic substances, including proteins and other biological substances; UV-VIS and IR spectroscopy; growth, imperfect structure and physical properties of crystals; thin films, liquid crystals, nanomaterials, partially disordered systems, and the methods of studies.
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