L. V. Smaglii, V. S. Gusakova, S. V. Gusakova, M. A. Pshemyskiy, S. O. Koshuba, E. A. Golovanov
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引用次数: 0
Abstract
We studied the effect of the purinergic signaling system and
the contribution of Cl– transporters
to isosmotic contraction of vascular smooth muscle cells (SMC),
which results from the normalization of osmotic pressure after prolonged
incubation in a hyposmotic medium. The study was carried out by myography
of endothelium-denuded aortic ring segments of male Wistar rats.
Isosmotic contraction was induced by placing vascular segments in
normosmotic Krebs solution (120 mM NaCl) after a 40-minute incubation
in a hyposmotic Krebs solution (40 mM NaCl). Purinergic receptors
were activated by adenosine 5’-triphosphate (ATP, 500 µM), a nonselective
P2X and P2Y receptor
agonist, and uridine 5’-triphosphate (UTP, 500 µM), a selective
P2Y receptor agonist. ATP and UTP abolished
the transience of the aortic SMC isosmotic contraction without affecting
its amplitude. Pretreatment of vascular segments with ATP and UTP
during incubation in a hyposmotic solution completely suppressed
the development of isosmotic contraction in the presence of ATP
or UTP, but did not affect it without purinergic receptor activators.
The Na+–K+–2Cl–-cotransporter
(NKCC) inhibitor bumetanide (100 µM) abolished isosmotic contraction
in the presence of ATP (not UTP) but restored its transience. The
nonselective Cl– channel and Cl–/HCO3– exchanger
blocker DIDS (100 µM) suppressed the development of isosmotic contraction
in the presence of both ATP and UTP. The potassium channel blocker tetraethylammonium
(10 mM) potentiated the contractile activity of UTP toward isosmotic
volume contraction. Presumably, purinergic receptors abolish the
transience of isosmotic contraction by activating Cl– currents
through activation of P2Y receptors. The
mechanism of interaction between the purinergic signaling system
and Cl– transport during cell volume
changes requires further investigation.
期刊介绍：
Journal of Evolutionary Biochemistry and Physiology publishes original experimental and theoretical and review articles related to evolution of the main forms of metabolism in connection with life origin; comparative and ontogenetic physiology and biochemistry, biochemical evolution of animal world; as well as evolution of functions; morphology, pharmacology, pathophysiology and ecological physiology. The journal welcomes manuscripts from all countries in the English or Russian language.
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