{"title":"Study of Na+/K+-ATPase and Components of the Ca2+-Transporting System in Myocardium under Experimental Prediabetes and Type 1 Diabetes in Rats","authors":"I. B. Sukhov, O. V. Chistyakova, M. G. Dobretsov","doi":"10.1134/s0022093024030232","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>One of the complications of diabetes mellitus (DM) is diabetic\ncardiomyopathy (DCM), whose molecular mechanisms of pathogenesis\nhave not been fully studied. Previously, the involvement of Na<sup>+</sup>/K<sup>+</sup>-ATPase\nand components of the Ca<sup>2+</sup> transport\nsystem in cardiomyocytes in the development of DCM was shown. The\naim of the work was to study the expression and activity of Na<sup>+</sup>/K<sup>+</sup>-ATPase\nand Ca<sup>2+</sup>-ATPase (SERCA2) in the myocardium\nof male Wistar rats in a model of streptozotocin (STZ)-induced prediabetes\nand overt type 1 diabetes (T1DM). STZ was administered at once i.p.\nin doses of 30–35 mg/kg. Rats with glucose levels above 11 mM were\nconsidered diabetic (STZ-D1 group), and those with moderate hyperglycemia\nwere considered prediabetic (STZ-preD1 group). The activity of Na<sup>+</sup>/K<sup>+</sup>-ATPase\nand Ca<sup>2+</sup>-ATPase was determined (by the\nrate of release of inorganic phosphate, P<sub>i</sub>), and\nthe expression of the genes α1- and α2-isoforms of Na<sup>+</sup>/K<sup>+</sup>-ATPase,\nSERCA2, and Kir6.1, Kv7.1, and Kv2.1 potassium channels was also\ndetermined. In the control (C) group, the activity of ouabain (1 mM)\n-sensitive Mg<sup>2+</sup>-dependent ATPase was\n6.03 ± 0.6 mmol Pi/g/h. In the STZ-D1 and STZ-preD1 groups, Na<sup>+</sup>/K<sup>+</sup>-ATPase\nactivity did not differ from group C. The level of gene expression\nof α1- and α2- subunits of Na<sup>+</sup>/K<sup>+</sup>-ATPase\nin the STZ-D1 group decreased by more than 45%, then both in the\nSTZ-preD1 group increased by 64 and 81%, which may indicate a high\nsensitivity of expression to insulinopenia. The activity of Ca<sup>2+</sup>-ATPase\nand the expression of the SERCA2 gene did not differ between the\ngroups, which might be because the 4-week period after STZ administration\nis not sufficient for the development of Ca<sup>2+</sup>-ATPase\ndeficiency in the rat heart. The level of expression of the genes\nof the potassium channel subtypes Kv2.1, Kir6.1, and Kv7.1 increased\nin the STZ-preD1 group, which may indicate a potential contribution\nof the studied potassium channel subtypes to the adaptation mechanism to\nmoderate hyperglycemia.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"19 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Evolutionary Biochemistry and Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0022093024030232","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
One of the complications of diabetes mellitus (DM) is diabetic
cardiomyopathy (DCM), whose molecular mechanisms of pathogenesis
have not been fully studied. Previously, the involvement of Na+/K+-ATPase
and components of the Ca2+ transport
system in cardiomyocytes in the development of DCM was shown. The
aim of the work was to study the expression and activity of Na+/K+-ATPase
and Ca2+-ATPase (SERCA2) in the myocardium
of male Wistar rats in a model of streptozotocin (STZ)-induced prediabetes
and overt type 1 diabetes (T1DM). STZ was administered at once i.p.
in doses of 30–35 mg/kg. Rats with glucose levels above 11 mM were
considered diabetic (STZ-D1 group), and those with moderate hyperglycemia
were considered prediabetic (STZ-preD1 group). The activity of Na+/K+-ATPase
and Ca2+-ATPase was determined (by the
rate of release of inorganic phosphate, Pi), and
the expression of the genes α1- and α2-isoforms of Na+/K+-ATPase,
SERCA2, and Kir6.1, Kv7.1, and Kv2.1 potassium channels was also
determined. In the control (C) group, the activity of ouabain (1 mM)
-sensitive Mg2+-dependent ATPase was
6.03 ± 0.6 mmol Pi/g/h. In the STZ-D1 and STZ-preD1 groups, Na+/K+-ATPase
activity did not differ from group C. The level of gene expression
of α1- and α2- subunits of Na+/K+-ATPase
in the STZ-D1 group decreased by more than 45%, then both in the
STZ-preD1 group increased by 64 and 81%, which may indicate a high
sensitivity of expression to insulinopenia. The activity of Ca2+-ATPase
and the expression of the SERCA2 gene did not differ between the
groups, which might be because the 4-week period after STZ administration
is not sufficient for the development of Ca2+-ATPase
deficiency in the rat heart. The level of expression of the genes
of the potassium channel subtypes Kv2.1, Kir6.1, and Kv7.1 increased
in the STZ-preD1 group, which may indicate a potential contribution
of the studied potassium channel subtypes to the adaptation mechanism to
moderate hyperglycemia.
期刊介绍：
Journal of Evolutionary Biochemistry and Physiology publishes original experimental and theoretical and review articles related to evolution of the main forms of metabolism in connection with life origin; comparative and ontogenetic physiology and biochemistry, biochemical evolution of animal world; as well as evolution of functions; morphology, pharmacology, pathophysiology and ecological physiology. The journal welcomes manuscripts from all countries in the English or Russian language.
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