Endoglin Regulates Intercellular Interactions between Trophoblast and Natural Killer Cells

IF 0.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Evolutionary Biochemistry and Physiology Pub Date : 2024-06-26 DOI:10.1134/s0022093024030074

E. Tyshchuk, P. Grebenkina, I. Krutetskaya, I. Smirnov, A. Stolbovaya, O. Shashkova, M. Samoilovich, D. Bazhenov, O. Stepanova, S. Selkov, D. Sokolov

{"title":"Endoglin Regulates Intercellular Interactions between Trophoblast and Natural Killer Cells","authors":"E. Tyshchuk, P. Grebenkina, I. Krutetskaya, I. Smirnov, A. Stolbovaya, O. Shashkova, M. Samoilovich, D. Bazhenov, O. Stepanova, S. Selkov, D. Sokolov","doi":"10.1134/s0022093024030074","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The interaction of natural killer and trophoblast cells underlies\nmaternal-fetal immune tolerance. The data on the participation of\nendoglin (ENG, CD105), or its soluble form, in the regulation of\nthe communication of these cells are currently insufficient. In\nthis study, we have investigated the role of endoglin in the intercellular\ninteractions between natural killer cells and trophoblasts. Here,\nwe show that NK-92 cells and JEG-3 cells constitutively express\nMICA/B, and CD105. In the presence of JEG-3 cells, the expression\nof NKG2D, CD94, MICA/B, and CD105 by NK-92 cells was increased,\nand the number of NK-92 cells expressing NKG2A, CD94, and MICA was\nreduced. Antibodies against ENG and recombinant endoglin (rENG),\nattenuated the trophoblasts’ influence and returned the phenotype of\nthe NK-92 cells to that of cells found in monoculture conditions.\nThe antibodies and rENG also increased the expression of pSMAD2/3\nby NK cells in both monoculture and co-culture conditions. The antibodies\nincreased the trophoblasts’ sensitivity to the cytotoxic effect\nof NK cells. In general, our findings indicate a significant role\nof endoglin in the intercellular communication between NK cells\nand trophoblasts. We also speculate that endoglin forms a complex\nwith TGFβ, which aids in TGFβ trafficking between these cells.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"75 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Evolutionary Biochemistry and Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0022093024030074","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The interaction of natural killer and trophoblast cells underlies maternal-fetal immune tolerance. The data on the participation of endoglin (ENG, CD105), or its soluble form, in the regulation of the communication of these cells are currently insufficient. In this study, we have investigated the role of endoglin in the intercellular interactions between natural killer cells and trophoblasts. Here, we show that NK-92 cells and JEG-3 cells constitutively express MICA/B, and CD105. In the presence of JEG-3 cells, the expression of NKG2D, CD94, MICA/B, and CD105 by NK-92 cells was increased, and the number of NK-92 cells expressing NKG2A, CD94, and MICA was reduced. Antibodies against ENG and recombinant endoglin (rENG), attenuated the trophoblasts’ influence and returned the phenotype of the NK-92 cells to that of cells found in monoculture conditions. The antibodies and rENG also increased the expression of pSMAD2/3 by NK cells in both monoculture and co-culture conditions. The antibodies increased the trophoblasts’ sensitivity to the cytotoxic effect of NK cells. In general, our findings indicate a significant role of endoglin in the intercellular communication between NK cells and trophoblasts. We also speculate that endoglin forms a complex with TGFβ, which aids in TGFβ trafficking between these cells.

Abstract Image

查看原文本刊更多论文

内胚层蛋白调控滋养层细胞与自然杀伤细胞之间的细胞间相互作用

摘要自然杀伤细胞和滋养层细胞的相互作用是母胎免疫耐受的基础。目前，有关内切蛋白（ENG，CD105）或其可溶性形式参与调节这些细胞间通讯的数据尚不充分。在这项研究中，我们探讨了内多吉林在自然杀伤细胞和滋养细胞之间的细胞间相互作用中的作用。在这里，我们发现 NK-92 细胞和 JEG-3 细胞组成性地表达 MICA/B 和 CD105。在 JEG-3 细胞存在的情况下，NK-92 细胞表达 NKG2D、CD94、MICA/B 和 CD105 的数量增加，而表达 NKG2A、CD94 和 MICA 的 NK-92 细胞数量减少。针对ENG的抗体和重组内切蛋白（rENG）减弱了滋养细胞的影响，使NK-92细胞的表型恢复到单培养条件下的表型。抗体增加了滋养细胞对 NK 细胞毒性作用的敏感性。总之，我们的研究结果表明，内切蛋白在NK细胞与滋养细胞之间的细胞间通讯中起着重要作用。我们还推测，内切蛋白与TGFβ形成复合物，有助于TGFβ在这些细胞间的运输。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Evolutionary Biochemistry and Physiology 生物-进化生物学

自引率

33.30%

发文量

110

审稿时长

6-12 weeks

期刊介绍： Journal of Evolutionary Biochemistry and Physiology publishes original experimental and theoretical and review articles related to evolution of the main forms of metabolism in connection with life origin; comparative and ontogenetic physiology and biochemistry, biochemical evolution of animal world; as well as evolution of functions; morphology, pharmacology, pathophysiology and ecological physiology. The journal welcomes manuscripts from all countries in the English or Russian language.