Cinchona officinalis L. ex situ conservation by in vitro slow growth and cryopreservation techniques

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Rosa Armijos-González, Pablo Ramón, Augusta Cueva-Agila
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Abstract

Cinchona officinalis has experienced anthropogenic pressures for nearly 400 years, such as overexploitation, habitat fragmentation, and the subsequent reduction of genetic diversity. Additionally, the challenge of regeneration in its natural environment makes it a vulnerable species. In this context, various treatments for the in vitro conservation of explants were evaluated in the present study. Conservation by slow growth, the effects of osmotic substances such as sorbitol, mannitol, and sucrose at different concentrations were assessed. Different concentrations of MS and B5 culture media were also examined for their impact on the growth, budding, mortality, and rooting of explants over 12 months without subcultures. For long-term conservation by cryopreservation, two techniques were tested: vitrification and encapsulation-dehydration. Short-term preservation of explants in sorbitol resulted in low mortality, minimal growth, and limited development of new shoots compared to preservation in sucrose or mannitol, although tissues could be recovered successfully from all storage conditions. After cryopreservation and 45 days of recovery, explants with the lowest mortality (4%) were from the control treatment (without cryoprotection) cultivated in a medium with sucrose which proved useful as a cryoprotectant. In conclusion, it is possible to conserve C. officinalis tissues in the short-term using in vitro techniques, while further assays are needed for long-term conservation.

Abstract Image

通过体外缓慢生长和低温保存技术对金鸡纳树(Cinchona officinalis L.)进行异地保存
金鸡纳树经历了近 400 年的人为压力,如过度开发、栖息地破碎化以及随之而来的遗传多样性减少。此外,在自然环境中再生的挑战也使其成为一个易危物种。在这种情况下,本研究对体外保存外植体的各种处理方法进行了评估。通过缓慢生长进行保存,评估了不同浓度的山梨醇、甘露醇和蔗糖等渗透物质的效果。此外,还研究了不同浓度的 MS 和 B5 培养基在 12 个月内对外植体生长、出芽、死亡率和生根的影响。对于低温长期保存,测试了两种技术:玻璃化和封装-脱水。与在蔗糖或甘露醇中保存外植体相比,在山梨醇中短期保存外植体的死亡率低、生长量极小、新芽发育有限,尽管组织可以从所有保存条件中成功恢复。经过低温保存和 45 天的恢复,死亡率最低(4%)的外植体来自在蔗糖培养基中培养的对照处理(无低温保护),蔗糖被证明是一种有用的低温保护剂。总之,使用体外技术可以在短期内保存 C. officinalis 组织,而长期保存则需要进一步的试验。
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来源期刊
Plant Cell, Tissue and Organ Culture
Plant Cell, Tissue and Organ Culture 生物-生物工程与应用微生物
CiteScore
5.40
自引率
13.30%
发文量
203
审稿时长
3.3 months
期刊介绍: This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues. The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.
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