Evaluation of 21 different media on shoot regeneration in 11 cultivars of citrus using juvenile tissue

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Filipe Sathler Meira, Min Shao, Randall P. Niedz, James G. Thomson
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Abstract

Citrus is one of the most important global fruit crops, with oranges accounting for more than half the total production. Citrus can be genetically engineered for improved traits, but the process is severely limited by shoot regeneration rates, especially in commercial varieties. Although standardizing a transformation protocol is difficult due to genotype and season dependencies, use of an optimal mineral nutrient basal culture medium increases the success of recovering transgenic Citrus cells. The purpose of this study is to improve shoot regeneration from juvenile tissue in multiple Citrus genotypes. Explants of 11 Citrus cultivars were regenerated in 21 published media to determine the optimal media for each genotype. The number of shoots from juvenile tissue were counted 90 days after the explants were first cultured. The type of basal medium strongly affected the rates of shoot regeneration from Citrus juvenile tissue, and the effectiveness was negatively correlated with the level of ammonium. For each Citrus grouping, optimized media formulations were determined, and potential improvements were predicted. In general, medium R7100 appeared to be the most effective for regeneration of the Citrus genotypes tested.

Abstract Image

评估 21 种不同培养基对 11 个柑橘栽培品种幼枝再生的影响
柑橘是全球最重要的水果作物之一,其中橙子占总产量的一半以上。柑橘可以通过基因工程改良性状,但这一过程受到嫩枝再生率的严重限制,尤其是在商业品种中。虽然由于基因型和季节的依赖性,很难实现转化方案的标准化,但使用最佳的矿物质营养基础培养基可提高柑橘细胞转基因的成功率。本研究的目的是改善多种柑橘基因型的幼枝组织再生。11 个柑橘栽培品种的外植体在 21 种已发表的培养基中再生,以确定每种基因型的最佳培养基。在首次培养外植体 90 天后,对幼体组织的芽数进行计数。基础培养基的类型对柑橘幼苗组织的嫩枝再生率有很大影响,其效果与铵的含量呈负相关。针对每个柑橘组别,确定了优化的培养基配方,并预测了潜在的改进方案。总体而言,R7100 培养基似乎对测试的柑橘基因型的再生最有效。
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来源期刊
Plant Cell, Tissue and Organ Culture
Plant Cell, Tissue and Organ Culture 生物-生物工程与应用微生物
CiteScore
5.40
自引率
13.30%
发文量
203
审稿时长
3.3 months
期刊介绍: This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues. The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.
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