Assessment of synergism in combinations of essential oils against cattle tick Rhipicephalus microplus (Acari: Ixodidae)

IF 1.8 2区农林科学 Q2 ENTOMOLOGY

Experimental and Applied Acarology Pub Date : 2024-06-27 DOI:10.1007/s10493-024-00937-0

Lalnunpari Vangchhia, Jyoti ., Harkirat Singh, Vinod Kumar Dumka, Nirbhay K. Singh

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Abstract

Rhipicephalus microplus (Acari: Ixodidae) is a highly invasive tick responsible for significant economic losses to cattle industry worldwide. The increasing global reports on acaricide resistant tick populations warrant development of alternate eco-friendly approaches for suppressing the tick populations and vectored pathogens. The present study aimed to evaluate the acaricidal activity of essential oils (EOs): Cedar oil (CO), Garlic oil (GO), Peppermint oil (PO) and their combinations against R. microplus by larval packet test. Six concentrations each of individual EOs and their combinations (10, 5, 2.5, 1.25, 0.625 and 0.31%) were evaluated. The lethal concentrations (LC) of individual EOs and their combinations along with values of Combination Index (CI) and Dose Reduction Index (DRI) were estimated to assess the effects of interactions (synergistic, additive or antagonistic) of EO mixtures. Among the individual oils, GO showed the highest efficacy with lowest LC₅₀, LC₉₀ and LC₉₅ (95% CL) values at 2.19% (1.96–2.43%), 4.00% (3.48–4.98%) and 4.74% (4.01–6.23%), respectively. Among combinations, highest acaricidal property was exhibited by GO + PO (LC₅₀, LC₉₀ and LC₉₅ values of 2.63, 4.87 and 5.81%) with CI value of 0.598 indicating moderate synergism, while the CO + GO + PO combination (LC₅₀, LC₉₀ and LC₉₅ values of 1.67, 9.97 and 16.54%) exhibited slight synergism with CI value at 0.872 and favourable DRI of 8.24, 1.85 and 4.76 for PO, GO and CO, respectively. The present study seems to be pioneer to assess the combination effect of these EOs against R. microplus and could help in development of an effective and eco-friendly product for tick control.

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评估精油组合对牛蜱 Rhipicephalus microplus（Acari: Ixodidae）的协同作用

Rhipicephalus microplus（Acari：Ixodidae）是一种高度入侵性蜱虫，给全球养牛业造成了重大经济损失。全球有关蜱虫对杀螨剂产生抗药性的报道越来越多，因此需要开发其他生态友好型方法来抑制蜱虫种群和媒介病原体。本研究旨在评估精油（EOs）的杀螨活性：雪松油（CO）、大蒜油（GO）、薄荷油（PO）及其复方精油（EO）通过幼虫包测试对小蜱具有杀螨活性。评估了六种浓度的单个 EO 及其组合（10%、5%、2.5%、1.25%、0.625% 和 0.31%）。估算了单个环氧乙烷及其组合的致死浓度（LC）以及组合指数（CI）和剂量降低指数（DRI）值，以评估环氧乙烷混合物的相互作用（协同作用、相加作用或拮抗作用）效果。在单种油中，GO 的功效最高，最低 LC50、LC90 和 LC95（95% CL）值分别为 2.19% (1.96-2.43%)、4.00% (3.48-4.98%) 和 4.74% (4.01-6.23%)。CO + GO + PO 组合（LC50、LC90 和 LC95 值分别为 1.67、9.97 和 16.54%）表现出轻微的协同作用，CI 值为 0.872，PO、GO 和 CO 的有利 DRI 分别为 8.24、1.85 和 4.76。本研究似乎是评估这些环氧乙烷对 R. microplus 的综合效果的先驱，有助于开发一种有效、环保的蜱虫防治产品。

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来源期刊

Experimental and Applied Acarology 生物-昆虫学

CiteScore

3.90

自引率

9.10%

发文量

审稿时长

4-8 weeks

期刊介绍： Experimental and Applied Acarology publishes peer-reviewed original papers describing advances in basic and applied research on mites and ticks. Coverage encompasses all Acari, including those of environmental, agricultural, medical and veterinary importance, and all the ways in which they interact with other organisms (plants, arthropods and other animals). The subject matter draws upon a wide variety of disciplines, including evolutionary biology, ecology, epidemiology, physiology, biochemistry, toxicology, immunology, genetics, molecular biology and pest management sciences.