Yujie Wen, Cong Tang, Qing Shen, Shuqing Dong, Yaya Wang, Yunchun Li and Shijun Shao
{"title":"An efficient ratiometric fluorescence and colorimetric dual-mode probe for convenient determination of nitrite in real samples and E. coli†","authors":"Yujie Wen, Cong Tang, Qing Shen, Shuqing Dong, Yaya Wang, Yunchun Li and Shijun Shao","doi":"10.1039/D4AY00417E","DOIUrl":null,"url":null,"abstract":"<p >Developing an effective and convenient nitrite detection method is indispensable in food safety, environmental monitoring, clinical diagnosis of diseases, and many other areas. Herein, a dicyanoisophorone derivative, <strong>TMN-NH<small><sub>2</sub></small></strong> with large Stokes shift and near-infrared (NIR) emission, was proposed as a ratiometric fluorescence and colorimetric dual-mode probe for the rapid determination of NO<small><sub>2</sub></small><small><sup>−</sup></small> in acidic media, showing excellent selectivity and high sensitivity. The sensing mechanism is based on the diazotization of <strong>TMN-NH<small><sub>2</sub></small></strong> with NO<small><sub>2</sub></small><small><sup>−</sup></small> and subsequent diazonium salt hydrolysis to form a hydroxyl-substituted product (<strong>TMN-OH</strong>). Under the optimized conditions of reaction and detection, a new quantitative analysis method based on <strong>TMN-NH<small><sub>2</sub></small></strong> was established for NO<small><sub>2</sub></small><small><sup>−</sup></small> detection, exhibiting good linear relationships to NO<small><sub>2</sub></small><small><sup>−</sup></small> in the range of 0.5 to 15 μM with practical detection limits of 26.6 nM and 17.6 nM for the colorimetric and fluorescent readout, respectively. The quantitative detection of NO<small><sub>2</sub></small><small><sup>−</sup></small> in real samples demonstrated satisfactory recoveries and repeatability. Moreover, <strong>TMN-NH<small><sub>2</sub></small></strong> was successfully applied for monitoring NO<small><sub>2</sub></small><small><sup>−</sup></small> in <em>Escherichia coli</em> by confocal fluorescence imaging.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Methods","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/ay/d4ay00417e","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Developing an effective and convenient nitrite detection method is indispensable in food safety, environmental monitoring, clinical diagnosis of diseases, and many other areas. Herein, a dicyanoisophorone derivative, TMN-NH2 with large Stokes shift and near-infrared (NIR) emission, was proposed as a ratiometric fluorescence and colorimetric dual-mode probe for the rapid determination of NO2− in acidic media, showing excellent selectivity and high sensitivity. The sensing mechanism is based on the diazotization of TMN-NH2 with NO2− and subsequent diazonium salt hydrolysis to form a hydroxyl-substituted product (TMN-OH). Under the optimized conditions of reaction and detection, a new quantitative analysis method based on TMN-NH2 was established for NO2− detection, exhibiting good linear relationships to NO2− in the range of 0.5 to 15 μM with practical detection limits of 26.6 nM and 17.6 nM for the colorimetric and fluorescent readout, respectively. The quantitative detection of NO2− in real samples demonstrated satisfactory recoveries and repeatability. Moreover, TMN-NH2 was successfully applied for monitoring NO2− in Escherichia coli by confocal fluorescence imaging.