Diamond-based quantum sensing of free radicals in migrating human breast cancer cells

IF 10.5 2区材料科学 Q1 CHEMISTRY, PHYSICAL

Carbon Pub Date : 2024-06-30 DOI:10.1016/j.carbon.2024.119405

Claudia Reyes-San-Martin , Arturo Elías-Llumbet , Elkin Escobar-Chaves , Marcia Manterola , Aldona Mzyk , Romana Schirhagl

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引用次数: 0

Abstract

Cell migration is a crucial parameter for disease progression in cancer. Reactive oxygen species (ROS) levels are involved in the regulation of the migration process, however, the exact role of free radical generation and where it occurs is unknown. Here we use a diamond-based quantum sensing technique to detect free radicals during cancer cell migration in real time with subcellular resolution. We investigated metastatic MDA-MB-231 human breast cancer cells and observed free radical formation after 16 h of starvation and 24 h of migration under low-serum conditions. Intracellular diamond dynamics were monitored at different migration points (0, 12, and 24 h), and cell morphology was evaluated. Additionally, the number of focal adhesions was analyzed as an indicator of the migratory potential of the cells. We further measured free radical generation under nicotinamide adenine dinucleotide phosphate hydrogen NADPH oxidases 2 (NOX2) inhibition by apocynin. We found that free radical levels decreased after 24 h treatment with 36 μg/mL apocynin while the levels of ROS and the migratory capacity of the cells increased. Our results evidence the complexity of the redox regulation in migrating cancer cells and offer a novel approach to specifically and locally interrogate pivotal players of the oxidative network behind metastatic success.

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基于金刚石的自由基迁移人类乳腺癌细胞量子传感技术

细胞迁移是癌症疾病进展的一个关键参数。活性氧（ROS）水平参与了迁移过程的调控，但自由基产生的确切作用及其发生的位置尚不清楚。在这里，我们使用一种基于金刚石的量子传感技术，以亚细胞分辨率实时检测癌细胞迁移过程中的自由基。我们研究了转移性 MDA-MB-231 人类乳腺癌细胞，观察了在低血清条件下饥饿 16 小时和迁移 24 小时后自由基的形成。在不同的迁移点（0、12 和 24 小时）监测了细胞内钻石的动态变化，并对细胞形态进行了评估。此外，还分析了病灶粘连的数量，作为细胞迁移潜力的指标。我们进一步测量了阿朴昔宁抑制烟酰胺腺嘌呤二核苷酸磷酸氢盐 NADPH 氧化酶 2（NOX2）作用下自由基的生成情况。我们发现，在使用 36 μg/mL 阿朴霉素处理 24 小时后，自由基水平下降，而 ROS 水平和细胞迁移能力上升。我们的研究结果证明了癌细胞迁移过程中氧化还原调控的复杂性，并提供了一种新的方法来特异性地、局部地检测转移成功背后氧化网络中的关键角色。

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来源期刊

Carbon 工程技术-材料科学：综合

CiteScore

20.80

自引率

7.30%

发文量

审稿时长

23 days

期刊介绍： The journal Carbon is an international multidisciplinary forum for communicating scientific advances in the field of carbon materials. It reports new findings related to the formation, structure, properties, behaviors, and technological applications of carbons. Carbons are a broad class of ordered or disordered solid phases composed primarily of elemental carbon, including but not limited to carbon black, carbon fibers and filaments, carbon nanotubes, diamond and diamond-like carbon, fullerenes, glassy carbon, graphite, graphene, graphene-oxide, porous carbons, pyrolytic carbon, and other sp2 and non-sp2 hybridized carbon systems. Carbon is the companion title to the open access journal Carbon Trends. Relevant application areas for carbon materials include biology and medicine, catalysis, electronic, optoelectronic, spintronic, high-frequency, and photonic devices, energy storage and conversion systems, environmental applications and water treatment, smart materials and systems, and structural and thermal applications.