{"title":"Obtaining and Primary Phenotypic Characterization of Berlin Poplar Transformed by AtGA20ox1 Gene","authors":"V. V. Pavlichenko, M. V. Protopopova","doi":"10.1134/s1021443724604646","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Increasing the rate of biomass gain is one of the most important areas in the breeding of woody plants. However, the application of classical breeding approaches to woody plants is significantly limited due to the long breeding cycles of many species. The development of genetic engineering and genome editing technologies has made it possible to improve tree traits in a relatively short time. Genes for the biosynthesis of phytohormones are often the target of genetic manipulations aimed at accelerating plant growth. Gibberellin 20-oxidase is a key enzyme responsible for the active production of gibberellins in plants and is, therefore, a preferred target for genetic manipulation to increase growth rate. This paper presents an original study on the production of <i>Populus</i> <i>×</i> <i>berolinensis</i> K. Koch, transformed by gene encoding gibberellin 20-oxidase from <i>Arabidopsis thaliana</i> (L.) Heynh. (<i>AtGA20ox1</i>), and the initial evaluation of the phenotypic effects of the transformation. The main phenotypic manifestations of the transformation were a pronounced elongation of the stem due to an increase in the size of the internodes, a slight thinning of the stem, as well as lengthening and narrowing of the leaves. Our results showed that <i>AtGA20ox1</i> gene overexpression in Berlin poplar leads to an acceleration of its growth by at least three times under in vitro conditions compared to control values. The negative effects of transformation, expressed in weak rooting or a high frequency of apical necrosis and observed in some transgenic strains at the initial stages of selection, do not appear in the three final selected strains.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"30 1","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Russian Journal of Plant Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s1021443724604646","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Increasing the rate of biomass gain is one of the most important areas in the breeding of woody plants. However, the application of classical breeding approaches to woody plants is significantly limited due to the long breeding cycles of many species. The development of genetic engineering and genome editing technologies has made it possible to improve tree traits in a relatively short time. Genes for the biosynthesis of phytohormones are often the target of genetic manipulations aimed at accelerating plant growth. Gibberellin 20-oxidase is a key enzyme responsible for the active production of gibberellins in plants and is, therefore, a preferred target for genetic manipulation to increase growth rate. This paper presents an original study on the production of Populus×berolinensis K. Koch, transformed by gene encoding gibberellin 20-oxidase from Arabidopsis thaliana (L.) Heynh. (AtGA20ox1), and the initial evaluation of the phenotypic effects of the transformation. The main phenotypic manifestations of the transformation were a pronounced elongation of the stem due to an increase in the size of the internodes, a slight thinning of the stem, as well as lengthening and narrowing of the leaves. Our results showed that AtGA20ox1 gene overexpression in Berlin poplar leads to an acceleration of its growth by at least three times under in vitro conditions compared to control values. The negative effects of transformation, expressed in weak rooting or a high frequency of apical necrosis and observed in some transgenic strains at the initial stages of selection, do not appear in the three final selected strains.
期刊介绍：
Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.
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