Foliar Application of Chitosan and Salicylic Acid Improved Milk Thistle’s Tolerance to Drought Stress

IF 1.1 4区 生物学 Q3 PLANT SCIENCES
R. Ghanbari Moheb Seraj, M. Behnamian, A. Ahmadikhah, K. Esmaeilzadeh-Salestani
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Abstract

Drought is the main abiotic agent for growth limiters and crop production. Therefore, in this study, we investigated the effect of chitosan and salicylic acid on morphological and physiological parameters and fatty acids content under drought stress. The first factor was drought stress levels (Field capacity (F.C), 70% of F.C and 40% of F.C), the second factor was elicitors (control (C), 0.5 mM salicylic acid, 1 mM salicylic acid, 100 mg/L chitosan and 200 mg/L chitosan) and the third factor was sampling times (3, 7, 14, 21 days after drought stress) with three replications. According to the results, comparing the three drought stress levels, severe stress treatment (40% F.C) significantly reduced the growth traits of milk thistle. Foliar application of chitosan and salicylic acid significantly compensated for the reduction of growth traits in plants under drought stress, in which chitosan frequently had higher growth and tolerance than salicylic acid. The role of elicitors in reducing the stress negative effects on plant growth is often due to stimulating chlorophylls, reducing hydrogen peroxide levels, and production of antioxidant enzymes. In our findings, chitosan demonstrated an increase of 28.4, 10, and 8% in relative water content (RWC), chlorophylls, and catalase activity, respectively, compared to the control. Similarly, salicylic acid revealed an increase of 29.3, 4, and 2% in the same parameters compared to the control. Besides, hydrogen peroxide in plants treated with chitosan and salicylic acid decreased by 20 and 15%, respectively. Besides, drought stress significantly reduced the predominant fatty acids in the seed, which elicitor application reduced the effect of stress slightly. Chitosan had a higher effect than salicylic acid in increasing fatty acids content. Foliar application of CHT and SA increased plant tolerance under drought stress. A comparison of two elicitors showed that CHT had a clear effect on plant development and amplification of defense systems against SA.

Abstract Image

叶面喷施壳聚糖和水杨酸提高了奶蓟草对干旱胁迫的耐受性
摘要 干旱是限制作物生长和提高作物产量的主要非生物因素。因此,本研究探讨了壳聚糖和水杨酸在干旱胁迫下对形态、生理参数和脂肪酸含量的影响。研究的第一个因素是干旱胁迫水平(田间能力(F.C)、70% F.C和40% F.C),第二个因素是诱导剂(对照(C)、0.5 mM水杨酸、1 mM水杨酸、100 mg/L壳聚糖和200 mg/L壳聚糖),第三个因素是取样时间(干旱胁迫后3、7、14、21天),共3次重复。结果表明,比较三种干旱胁迫水平,严重胁迫处理(40% F.C)显著降低了奶蓟草的生长性状。叶面喷施壳聚糖和水杨酸能明显补偿干旱胁迫下植物生长性状的降低,其中壳聚糖的生长和耐受性常常高于水杨酸。激发剂在减少胁迫对植物生长的负面影响方面的作用通常是通过刺激叶绿素、降低过氧化氢水平和产生抗氧化酶来实现的。我们的研究结果表明,与对照组相比,壳聚糖的相对含水量(RWC)、叶绿素和过氧化氢酶活性分别提高了 28.4%、10% 和 8%。同样,与对照相比,水杨酸在相同参数上分别增加了 29.3%、4% 和 2%。此外,经壳聚糖和水杨酸处理的植物过氧化氢分别减少了 20% 和 15%。此外,干旱胁迫明显降低了种子中主要脂肪酸的含量,施用诱导剂略微减轻了胁迫的影响。在增加脂肪酸含量方面,壳聚糖的效果高于水杨酸。叶面喷施 CHT 和 SA 提高了植物在干旱胁迫下的耐受性。对两种诱导剂的比较表明,CHT 对植物的生长发育有明显的影响,并能增强植物对 SA 的防御系统。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.00
自引率
14.30%
发文量
107
审稿时长
6 months
期刊介绍: Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.
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