T. Jafari, Alireza Iranbakhsh, K. Kamali Aliabad, F. Daneshmand, S. E. Seifati
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引用次数: 0
Abstract
This study explored whether the exogenously applied nitric oxide (NO; 25 µM for 12 times with 7 days intervals) triggers variations in growth, physiological traits, and molecular characteristics in quinoa Chenopodium quinoa seedlings under two electrical conductivity (EC) conditions, including 1.5 and 8 dS/m. The foliar application of NO not only increased the number of leaves produced and the biomass of the shoots under the low EC conditions, but also mitigated the risk associated with the high EC conditions. Higher proline levels were recorded in both the leaves and the roots of plants treated with NO and/or salinity among which the (NO + salinity) group had the highest amount. Both NO and high EC treatments contributed to the reduction of the saponin metabolite concentration. The highest activity of the PAL enzyme was recorded in NO-treated seedlings cultivated under high EC conditions. Total soluble phenolic and flavonoid content exhibited a similar trend to that of the PAL activity in response to the NO treatments under two EC conditions. The NO or saline treatments individually up-regulated the WRKY transcription factor by an average of 4.2-fold, while the NO treatment under the saline medium led to a drastic increase (9.7-fold) in the expression of this gene. The bZIP gene also showed a similar trend as the WRKY transcription factor gene. According to the statistical analysis, the saponin content was negatively correlated with the expression of the evaluated genes (WRKY and bZIP). In conclusion, NO confers salinity resistance and may improve the quality of quinoa-based foods by reducing saponin accumulation.
摘要 本研究探讨了外源施用一氧化氮（NO；25 µM，12次，每次间隔7天）是否会引发藜麦幼苗在两种电导率（EC）条件下（包括1.5和8 dS/m）的生长、生理性状和分子特征的变化。在低导电率条件下，叶面喷施 NO 不仅能增加叶片数量和嫩枝生物量，还能降低高导电率条件下的相关风险。经 NO 和/或盐度处理的植物，其叶片和根部的脯氨酸含量都较高，其中 NO + 盐度组的脯氨酸含量最高。氮氧化物和高欧共体处理都有助于降低皂素代谢物的浓度。在高导电率条件下培育的经 NO 处理的幼苗中，PAL 酶的活性最高。在两种 EC 条件下，总可溶性酚类和类黄酮含量与 PAL 活性对 NO 处理的反应趋势相似。NO 或盐水处理单独上调了 WRKY 转录因子，平均上调幅度为 4.2 倍，而在盐水培养基下 NO 处理导致该基因表达量剧增（9.7 倍）。bZIP 基因也表现出与 WRKY 转录因子基因类似的趋势。根据统计分析，皂素含量与评估基因（WRKY 和 bZIP）的表达呈负相关。总之，NO 可赋予藜麦抗盐能力，并可通过减少皂素积累来提高藜麦食品的质量。
期刊介绍：
Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.
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