Rapid eDNA survey reveals a unique biodiversity hotspot: The Corubal River, West Africa

IF 7.6 1区生物学 Q1 BIOLOGY

BioScience Pub Date : 2024-06-27 DOI:10.1093/biosci/biae036

Manuel Lopes-Lima, Vincent Prié, Mutaro Camará, Luis M P Ceríaco, Vasco Fernandes, Sónia Ferreira, Duarte V Gonçalves, Miguel Lecoq, Filipa M S Martins, Diogo Parrinha, Aissa Regalla de Barros, Alice Valentini, Joana Veríssimo, Luís Palma, Pedro Beja

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引用次数: 0

Abstract

The Corubal (Guinea-Bissau) is a wild but underexplored river in West Africa. This study underscores the potential of environmental DNA (eDNA) surveys to fill biodiversity knowledge gaps in the region. We filtered large water volumes at 11 sites along the watershed, amplified multiple molecular markers, and performed high PCR (polymerase chain reaction) replication and in-depth sequencing. We recorded 2589 amplicon sequence variants, with accumulation curves indicating the need for additional sampling to achieve a thorough survey. The taxonomic assignments were constrained by the scarcity of genomic resources. We recorded 125 species of aquatic and terrestrial vertebrates, including 21 new to the country, predominantly fish (61.9%). Surprisingly, crocodiles were not detected, despite their known presence. There were 11 imperiled species, two of which are Critically Endangered (Western chimpanzee and the mussel Pleiodon ovatus). Our findings support the conservation importance of the Corubal, provide a baseline for future monitoring, and highlight the challenges and opportunities of eDNA surveys in remote tropical rivers.

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快速 eDNA 调查揭示了一个独特的生物多样性热点：西非科鲁巴河

科鲁巴河（几内亚比绍）是西非的一条野生河流，但开发不足。这项研究强调了环境 DNA（eDNA）调查在填补该地区生物多样性知识空白方面的潜力。我们在流域沿岸的 11 个地点过滤了大量的水，扩增了多个分子标记，并进行了高PCR（聚合酶链反应）复制和深度测序。我们记录了 2589 个扩增子序列变异，累积曲线表明需要进行更多取样才能实现全面调查。由于基因组资源稀缺，分类工作受到限制。我们记录了 125 种水生和陆生脊椎动物，包括 21 种该国新发现的物种，主要是鱼类（61.9%）。令人惊讶的是，我们没有发现鳄鱼，尽管众所周知它们的存在。有 11 种濒危物种，其中两种为极度濒危物种（西部黑猩猩和贻贝 Pleiodon ovatus）。我们的研究结果证明了科鲁巴河保护工作的重要性，为今后的监测工作提供了基线，并凸显了在偏远热带河流进行 eDNA 调查所面临的挑战和机遇。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BioScience 生物-生物学

CiteScore

14.10

自引率

2.00%

发文量

109

审稿时长

3 months

期刊介绍： BioScience is a monthly journal that has been in publication since 1964. It provides readers with authoritative and current overviews of biological research. The journal is peer-reviewed and heavily cited, making it a reliable source for researchers, educators, and students. In addition to research articles, BioScience also covers topics such as biology education, public policy, history, and the fundamental principles of the biological sciences. This makes the content accessible to a wide range of readers. The journal includes professionally written feature articles that explore the latest advancements in biology. It also features discussions on professional issues, book reviews, news about the American Institute of Biological Sciences (AIBS), and columns on policy (Washington Watch) and education (Eye on Education).