Cu2O/SBA-3 Possess Intrinsic High Protease-Like Activity for Efficient Hydrolysis of Protein Under Physiological Conditions

IF 2.3 4区化学 Q3 CHEMISTRY, PHYSICAL

Catalysis Letters Pub Date : 2024-07-03 DOI:10.1007/s10562-024-04747-2

Daomei Chen, Guo Xiao, Jian Tang, Lixia zhao, Sicong Li, Lingli Li, Bin Li, Tao Lei, Jiaqiang Wang

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Abstract

Developing efficient artificial proteases still remains a great challenge due to the high stability of peptide bonds. Nanozymes have attracted great attention for their high stability, low cost and inherent physicochemical properties, providing new opportunities to break through natural enzyme inherent limitations, but the intrinsic mimic proteases properties of nanomaterials were seldom explored. Herein, we describe for the first time that SBA-3 supported Cu2O (Cu2O/SBA-3) exhibited excellent protease-like activity to hydrolysis of bovine serum albumin (BSA) and casein under neutral conditions, which is even superior to natural proteases (trypsin) and most of the other protease mimics under identical conditions. It exhibited surprisingly high catalytic activity and possessed good stability. As the first example of protease mimics consist of metallic compounds and mesoporous materials, Cu2O/SBA-3 has many advantages, such as easy preparation and separation, high activity and stability, mild reaction conditions, which makes this catalytic system have multiple of potential applications in biological systems.

Graphical Abstract

Abstract Image

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Cu2O/SBA-3 具有内在的高蛋白酶样活性，可在生理条件下高效水解蛋白质

由于肽键的高稳定性，开发高效的人工蛋白酶仍然是一项巨大的挑战。纳米酶因其高稳定性、低成本和固有的理化特性而备受关注，为突破天然酶的固有限制提供了新的机遇，但纳米材料的内在模拟蛋白酶特性却很少被探索。在此，我们首次描述了 SBA-3 支持的 Cu2O（Cu2O/SBA-3）在中性条件下水解牛血清白蛋白（BSA）和酪蛋白时表现出的类似蛋白酶的优异活性，甚至优于相同条件下的天然蛋白酶（胰蛋白酶）和大多数其他蛋白酶模拟物。它表现出惊人的高催化活性和良好的稳定性。作为首例由金属化合物和介孔材料组成的蛋白酶模拟物，Cu2O/SBA-3 具有易于制备和分离、高活性和稳定性、反应条件温和等诸多优点，这使得该催化体系在生物系统中具有多种潜在应用。

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来源期刊

Catalysis Letters 化学-物理化学

CiteScore

5.70

自引率

3.60%

发文量

327

审稿时长

1 months

期刊介绍： Catalysis Letters aim is the rapid publication of outstanding and high-impact original research articles in catalysis. The scope of the journal covers a broad range of topics in all fields of both applied and theoretical catalysis, including heterogeneous, homogeneous and biocatalysis. The high-quality original research articles published in Catalysis Letters are subject to rigorous peer review. Accepted papers are published online first and subsequently in print issues. All contributions must include a graphical abstract. Manuscripts should be written in English and the responsibility lies with the authors to ensure that they are grammatically and linguistically correct. Authors for whom English is not the working language are encouraged to consider using a professional language-editing service before submitting their manuscripts.