On the coupling of intracellular K+ ${{\rm{K}}}^{+}$ to glycolytic oscillations in yeast

IF 2.2 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yeast Pub Date : 2024-06-21 DOI:10.1002/yea.3972
Lars F. Olsen, Anita Lunding
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Abstract

We have investigated the interplay between glycolytic oscillations and intracellular concentration in the yeast Saccharomyces cerevisiae. Intracellular concentration was measured using the fluorophore potassium‐binding benzofuranisophthalate (PBFI). We found that is an essential ion for the occurrence of glycolytic oscillations and that intracellular concentration oscillates synchronously with other variables such as nicotinamide adenine dinucleotide hydride (NADH), intracellular adenosine triphosphate (ATP), and mitochondrial membrane potential. We also investigated if glycolysis and intracellular concentration oscillate in a number of yeast strains with mutations in transporters in the plasma membrane, mitochondrial membrane and in the vacuolar membrane. Most of these strains are still capable of showing glycolytic oscillations, but two strains are not: (i) a strain with a deletion in the mitochondrial Mdm38p transporter and (ii) a strain with deletion of the late endosomal Nhx1p () transporter. In these two mutant strains intracellular concentration seems to be low, indicating that the two transporters may be involved in transport of into the cytosol. In the strain, Mdm38p oscillations in glycolysis could be restored by addition of the exchange ionophore nigericin. Furthermore, in two nonoscillating mutant strains with a defective V‐ATPase and deletion of the Arp1p protein the intracellular is relatively high, suggesting that the V‐ATPase is essential for transport of out of the cytosol and that the cytoskeleton may be involved in binding to reduce the concentration of free ion in the cytosol. Analyses of the time series of oscillations of NADH, ATP, mitochondrial membrane potential, and potassium concentration using data‐driven modeling corroborate the conjecture that ion is essential for the emergence of oscillations and support the experimental findings using mutant strains.
细胞内 K+ ${{\rm{K}}^{+}$ 与酵母中糖酵解振荡的耦合关系
我们研究了酿酒酵母中糖酵解振荡与细胞内浓度之间的相互作用。我们使用荧光团苯并呋喃异酞酸钾(PBFI)测量了细胞内浓度。我们发现,细胞内浓度是发生糖酵解振荡的必要离子,而且细胞内浓度与烟酰胺腺嘌呤二核苷酸氢化物(NADH)、细胞内三磷酸腺苷(ATP)和线粒体膜电位等其他变量同步振荡。我们还研究了一些质膜、线粒体膜和液泡膜上的转运体发生突变的酵母菌株是否会发生糖酵解和细胞内浓度振荡。这些菌株中的大多数仍能表现出糖酵解振荡,但有两个菌株不能:(i) 线粒体 Mdm38p 转运体缺失的菌株;(ii) 内体晚期 Nhx1p()转运体缺失的菌株。在这两个突变株中,细胞内浓度似乎很低,表明这两种转运体可能参与了向细胞质的转运。在该菌株中,糖酵解中的 Mdm38p 振荡可以通过添加交换离子诱导剂尼格列汀来恢复。此外,在两个具有 V-ATP 酶缺陷和 Arp1p 蛋白缺失的非振荡突变株中,细胞内的离子浓度相对较高,这表明 V-ATP 酶是将离子转运出细胞膜的关键,而细胞骨架可能参与结合以降低细胞膜中游离离子的浓度。利用数据驱动模型对 NADH、ATP、线粒体膜电位和钾浓度的振荡时间序列进行分析,证实了离子对振荡的出现至关重要的猜想,并支持利用突变株的实验结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Yeast
Yeast 生物-生化与分子生物学
CiteScore
5.30
自引率
3.80%
发文量
55
审稿时长
3 months
期刊介绍: Yeast publishes original articles and reviews on the most significant developments of research with unicellular fungi, including innovative methods of broad applicability. It is essential reading for those wishing to keep up to date with this rapidly moving field of yeast biology. Topics covered include: biochemistry and molecular biology; biodiversity and taxonomy; biotechnology; cell and developmental biology; ecology and evolution; genetics and genomics; metabolism and physiology; pathobiology; synthetic and systems biology; tools and resources
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