Physiological responses and transcriptomic profiles unveil pivotal genes and pathways implicated in nano-elicited in vitro shoot proliferation of Bambusa balcooa

IF 2.3 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Plant Cell, Tissue and Organ Culture Pub Date : 2024-06-25 DOI:10.1007/s11240-024-02812-7

Anita Kumari, Shubham Joshi, Aqib Iqbal Dar, Rohit Joshi

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Abstract

Bamboos are perennial, arborescent, monocarpic, and industrially important non-timber grasses that are used as a pristine source of inorganic nutrients. However, conventional vegetative propagation methods demonstrated inadequate multiplication potential. This study investigates how Bambusa balcooa’s in vitro growth, photosynthetic pigment content, and antioxidant capacity were affected by citrate- and cetyltrimethylammonium bromide-coated gold nanoparticles (AuNPs). Further, to unravel the regulatory mechanism underlying gold nano-elicitation and in vitro plant behavior, we conducted RNA sequencing of non-treated control, 400 µM citrate-AuNPs-treated, and 600 µM CTAB-AuNPs-treated plantlets. Numerous morphological, physiological, and biochemical parameters were observed to be variably impacted along the citrate- and CTAB-coated AuNPs concentration gradient (200–600 µM). B. balcooa in vitro shoots supplemented with Murashige and Skoog medium enriched with 6-benzylaminopurine, naphthaleneacetic acid, and 400 µM citrate-AuNPs displayed statistically significant shoot proliferation, photosynthetic pigment accumulation, and antioxidant activities. Contrarily, a decline in growth parameters was observed in MS media supplemented with BAP, NAA, and 600 µM CTAB-AuNPs. Transcriptome profiling revealed various differentially expressed genes (DEGs) and metabolic pathways associated with nano-elicitation and plant growth. Furthermore, identifying genes (such as Glyoxalase, Expansin, and ZAT) governing in vitro proliferation and oxidative stress responses could enhance our understanding of the mechanisms underlying AuNPs’ ability to modulate various physiological and biochemical activities during micropropagation. Therefore, gene ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and the exploration of DEGs involved in the in vitro modulations regulated by AuNPs offer novel insights into the molecular mechanisms governing nano-elicited plant organogenesis more comprehensively.

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生理反应和转录组图谱揭示了纳米诱导簕杜鹃体外嫩枝增殖的关键基因和途径

竹子是多年生、乔木、单果，在工业上是重要的非木材禾本科植物，被用作无机营养的原始来源。然而，传统的无性繁殖方法显示其繁殖潜力不足。本研究探讨了柠檬酸盐和十六烷基三甲基溴化铵包覆的金纳米粒子（AuNPs）如何影响簕杜鹃的体外生长、光合色素含量和抗氧化能力。此外，为了揭示金纳米诱导和体外植物行为的调控机制，我们对未处理对照、400 µM 柠檬酸盐-AuNPs 处理和 600 µM CTAB-AuNPs 处理的小植株进行了 RNA 测序。沿着柠檬酸盐和 CTAB 涂层 AuNPs 浓度梯度（200-600 µM），观察到许多形态、生理和生化参数受到不同程度的影响。用富含 6-苄基氨基嘌呤、萘乙酸和 400 µM 柠檬酸盐-AuNPs 的 Murashige 和 Skoog 培养基补充的 B. balcooa 离体芽显示出显著的芽增殖、光合色素积累和抗氧化活性。相反，在添加了 BAP、NAA 和 600 µM CTAB-AuNPs 的 MS 培养基中观察到生长参数下降。转录组分析揭示了与纳米激发和植物生长相关的各种差异表达基因（DEG）和代谢途径。此外，确定调控体外增殖和氧化应激反应的基因（如糖醛酸酶、Expansin 和 ZAT）可加深我们对 AuNPs 在微繁殖过程中调节各种生理和生化活动的机制的理解。因此，基因本体（GO）富集、京都基因组百科全书（KEGG）通路分析以及参与AuNPs体外调控的DEGs的探索，为更全面地了解纳米诱导植物器官发生的分子机制提供了新的视角。

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来源期刊

Plant Cell, Tissue and Organ Culture 生物-生物工程与应用微生物

CiteScore

5.40

自引率

13.30%

发文量

203

审稿时长

3.3 months

期刊介绍： This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues. The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.