Induction and identification of polyploids in four Rhododendron species

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Xiaohuan Zhou, Peiting Zhao, Fanyu Zeng, Xingmin Geng, Jinliang Zhou, Jing Sun
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Abstract

To induce polyploidy in rhododendrons, different experimental materials of four Rhododendron species (2n = 2x = 26) were treated with colchicine and oryzalin as chemical mutagens. Three materials from R. fortunei, namely, seeds, cotyledons, and adventitious buds, were treated, and the highest tetraploid induction rate (15.64%) was observed in cotyledons treated with 20 mg·L− 1 oryzalin for 4 d. Two materials (stem tips and stem bases) were treated in R. simsii, and the highest tetraploid induction rate (4.04%) was achieved with 0.14% colchicine after soaking the stem tips for 48 h. However, when we treated the stem bases, the chimerism occurrence rate was up to 30.00%. In addition, polyploidy induction in R. ovatum was carried out at two colchicine concentrations (0.12 and 0.14%) with two materials (stem tip and stem base), which resulted in a higher chimeric rate. Three materials (stem tip, stem base, and adventitious buds) of R. molle were used, but no polyploidy was found. Polyploid identification was performed through stomatal observation and flow cytometry. Polyploid plants had larger and sparser stomata than diploid plants. Furthermore, morphological measurements revealed that the size of the stomata and leaves in tetraploid plants was larger than that in diploid plants. Stomatal density was decreased in tetraploid plants compared to diploid plants. Flow cytometry analysis identified tetraploids in the mutant plants of R. fortunei and R. simsii. Phenotypic analyses showed that tetraploid plants had smaller, rounder leaves and darker leaf colors than diploid plants. In summary, we compared the induced polyploid rhododendrons using different methods, which is of great significance for cultivating new rhododendron varieties with good ornamental properties and high resistance and for developing the rhododendron industry in China.

Abstract Image

四种杜鹃花多倍体的诱导和鉴定
为了诱导杜鹃花的多倍体,用秋水仙素和奥利唑啉作为化学诱变剂处理了四种杜鹃花(2n = 2x = 26)的不同实验材料。用 20 mg-L- 1 oryzalin 处理子叶 4 d,观察到最高的四倍体诱导率(15.64%)。对 R. simsii 的两种材料(茎尖和茎基部)进行了处理,用 0.14% 的秋水仙素浸泡茎尖 48 小时后,四倍体诱导率最高(4.04%),但处理茎基部时,嵌合体发生率高达 30.00%。此外,在两种秋水仙素浓度(0.12% 和 0.14%)下用两种材料(茎尖和茎基部)诱导 R. ovatum 的多倍体,结果嵌合率较高。使用了三种 R. molle 材料(茎尖、茎基部和不定芽),但没有发现多倍体。多倍体的鉴定是通过气孔观察和流式细胞仪进行的。与二倍体植物相比,多倍体植物的气孔更大、更稀疏。此外,形态测量显示,四倍体植株的气孔和叶片比二倍体植株大。与二倍体植物相比,四倍体植物的气孔密度有所降低。流式细胞仪分析在 R. fortunei 和 R. simsii 的突变植株中发现了四倍体。表型分析表明,与二倍体植株相比,四倍体植株的叶片更小、更圆,叶色更深。综上所述,我们比较了不同方法诱导的杜鹃花多倍体,这对培育观赏性好、抗性强的杜鹃花新品种和发展我国杜鹃花产业具有重要意义。
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来源期刊
Plant Cell, Tissue and Organ Culture
Plant Cell, Tissue and Organ Culture 生物-生物工程与应用微生物
CiteScore
5.40
自引率
13.30%
发文量
203
审稿时长
3.3 months
期刊介绍: This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues. The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.
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