Lung-on-a-chip composed of styrene-butadiene-styrene nano-fiber/porous PDMS composite membranes with cyclic triaxial stimulation

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION
Yuru You, Changling Zhang, Zhixiang Guo, Feng Xu, Daoheng Sun, Junjie Xia, Songyue Chen
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引用次数: 0

Abstract

The physiological function of lung is strongly correlated with its unique structural microenvironment and mechanical stimulation. Most existing lung-on-a-chips (LOCs) do not replicate the key physiological structure and stimulation of human lung, reducing their reliability in application. In this study, a scaffold structure of a styrene-butadiene-styrene (SBS) nanofiber and porous honeycomb polydime-thylsiloxane (PDMS) composite membrane was developed to construct an alveolar air-blood barrier that mimics the alveolar characteristics of flexibility, cross-scale structure, and triaxial mechanical stimulation. By combining micro-fluidic and electrospinning technology, a biomimetic LOC with dynamic triaxial cyclic strain was realized. The composite membrane had a Young’s modulus of 0.54 ± 0.05 MPa and was capable of 8–12% strain at 1 kPa air pressure. We monocultured and co-cultured human non-small cell lung cancer cells stably expressing red fluorescent protein (A549-RFP) with human umbilical vein endothelial cell stably expressing green fluorescent protein (HUVECs-GFP) within the chip. A multi-layered structure of epithelial cell layer-basal layer-endothelial cell layer, similar to the air-blood barrier in vivo, was constructed. The LOC was proved to be an initial foundation for creating in vitro alveolar physiological models, and could be a potential platform for application in physiology, pathology, toxicology, drug screening, and customized medicine.

Abstract Image

由苯乙烯-丁二烯-苯乙烯纳米纤维/多孔 PDMS 复合膜组成的肺芯片受到循环三轴刺激
肺的生理功能与其独特的微环境结构和机械刺激密切相关。现有的肺芯片(LOCs)大多无法复制人体肺部的关键生理结构和刺激,降低了其应用的可靠性。本研究开发了一种由苯乙烯-丁二烯-苯乙烯(SBS)纳米纤维和多孔蜂窝状聚二甲基硅氧烷(PDMS)复合膜组成的支架结构,用于构建肺泡气血屏障,以模拟肺泡的柔韧性、跨尺度结构和三轴机械刺激特性。通过结合微流体和电纺丝技术,实现了具有动态三轴循环应变的仿生物 LOC。复合膜的杨氏模量为 0.54 ± 0.05 兆帕,在 1 千帕气压下可承受 8-12% 的应变。我们将稳定表达红色荧光蛋白(A549-RFP)的人非小细胞肺癌细胞与稳定表达绿色荧光蛋白(HUVECs-GFP)的人脐静脉内皮细胞分别单培养和共培养在芯片中。这样就构建了一个类似于体内气血屏障的上皮细胞层-基底层-内皮细胞层的多层结构。事实证明,LOC 是创建体外肺泡生理模型的初步基础,可作为生理学、病理学、毒理学、药物筛选和定制医学的潜在应用平台。
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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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