Activity Alterations of 37 Tyrosine Kinase Inhibitors Upon kINPen Plasma Exposure in A549 Lung Cancer Cells

IF 4.6 Q1 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
P. Schulan;M. Wende;S. Bekeschus;M. Lalk;K. Wende
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Abstract

Cold physical plasma shows promising preclinical results as an anticancer strategy. The technology generates a variety of reactive oxygen species (ROS) mediating gas plasma-induced effects in cells and tissues. On the cellular level, ROS can trigger oxidative stress-related responses. On the biomolecular level, ROS can introduce oxidative modifications, potentially leading to functional alterations. To better understand plasma treatment in oncology therapies, we treated tyrosine kinase inhibitors (TKIs) with plasma to investigate the efficacy upon oxidation as well as plasma pretreated A549 lung cancer cells before TKI treatment to investigate combination effects. Specifically, a library of 37 compounds was exposed to the atmospheric pressure argon plasma jet kINPen before being added to the cells. Most gas plasma-treated TKIs showed a significant decline in anticancer efficacy. The experimental compound NVP-AEW541 showed elevated tumor-toxic effects after exposure to gas plasma. In A549 cells pretreated with gas plasma, all TKIs but one showed additive toxicity. In summary, this first study on gas plasma treatment of TKIs and lung cancer cells in combination treatments revealed that direct gas plasma TKI treatment decreased the activity of most but not all compounds investigated, while gas plasma pretreated cells mostly showed additive toxicity in response to TKI exposure.
37 种酪氨酸激酶抑制剂在 A549 肺癌细胞中暴露于 kINPen 血浆后的活性变化
冷物理等离子体作为一种抗癌策略,在临床前已取得了可喜的成果。该技术会产生多种活性氧(ROS),介导气体等离子体在细胞和组织中的诱导效应。在细胞层面,ROS 可引发氧化应激相关反应。在生物分子层面,ROS 可引入氧化修饰,从而可能导致功能改变。为了更好地了解肿瘤疗法中的血浆治疗,我们用血浆处理酪氨酸激酶抑制剂(TKIs),研究氧化后的疗效,并在TKI治疗前用血浆预处理A549肺癌细胞,研究联合效应。具体来说,在将 37 种化合物加入细胞之前,先将它们暴露在常压氩气等离子喷射器 kINPen 中。大多数经气体等离子体处理的 TKI 抗癌效果明显下降。实验性化合物 NVP-AEW541 在暴露于气体等离子体后显示出更高的肿瘤毒性效应。在用气体等离子体预处理的 A549 细胞中,除了一种 TKIs 外,所有 TKIs 都显示出相加毒性。总之,这项关于气体等离子体处理 TKIs 和肺癌细胞联合治疗的首次研究表明,直接用气体等离子体处理 TKI 会降低大多数(而非所有)受试化合物的活性,而气体等离子体预处理细胞大多会对 TKI 暴露表现出相加毒性。
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来源期刊
IEEE Transactions on Radiation and Plasma Medical Sciences
IEEE Transactions on Radiation and Plasma Medical Sciences RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
CiteScore
8.00
自引率
18.20%
发文量
109
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