Anti-Inflammatory Effects of Paraprobiotic Lactiplantibacillus plantarum KU15122 in LPS-Induced RAW 264.7 Cells.

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Journal of microbiology and biotechnology Pub Date : 2024-07-28 Epub Date: 2024-06-03 DOI:10.4014/jmb.2404.04052
Hye-Won Lee, Hee-Su Jung, Na-Kyoung Lee, Hyun-Dong Paik
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引用次数: 0

Abstract

Inflammation is a biodefense mechanism that provides protection against painful conditions such as inflammatory bowel disease, other gastrointestinal problems, and irritable bowel syndrome. Paraprobiotics have probiotic characteristics of intestinal modulation along with merits of safety and stability. In this study, heat-killed Lactiplantibacillus plantarum KU15122 (KU15122) was investigated for its anti-inflammatory properties. KU15122 was subjected to heat-killed treatment for enhancement of its safety, and its concentration was set at 8 log CFU/mL for conducting different experiments. Nitric oxide production was most remarkably reduced in the KU15122 group, whereas it was increased in the LPS-treated group. In RAW 264.7 cells, KU15122 inhibited the expression of inducible nitric oxide synthase, cyclooxygenase-2, interleukin (IL)-1β, IL-6, and tumor necrosis factor-α. ELISA revealed that among the tested strains, KU15122 exhibited the most significant reduction in PGE2, IL-1β, and IL-6. Moreover, KU15122 inhibited various factors involved in the nuclear factor-kappa B, activator protein-1, and mitogen-activated protein kinase pathways. In addition, KU15122 reduced the generation of reactive oxygen species. The anti-inflammatory effect of KU15122 was likely attributable to the bacterial exopolysaccharides. Conclusively, KU15122 exhibits anti-inflammatory potential against inflammatory diseases.

植物乳杆菌 KU15122 在 LPS 诱导的 RAW 264.7 细胞中的抗炎作用
炎症是一种生物防御机制,可防止炎症性肠病、其他胃肠道问题和肠易激综合征等痛苦病症。副益生菌具有调节肠道的益生特性,同时还具有安全和稳定的优点。在本研究中,研究人员对热处理杀死的植物乳杆菌 KU15122(KU15122)的抗炎特性进行了调查。为提高 KU15122 的安全性,对其进行了热处理,并将其浓度设定为 8 log CFU/mL,以进行不同的实验。一氧化氮的产生在 KU15122 组明显减少,而在 LPS 处理组则有所增加。在 RAW 264.7 细胞中,KU15122 可抑制诱导型一氧化氮合酶、环氧化酶-2、白细胞介素(IL)-1β、IL-6 和肿瘤坏死因子-α 的表达。酶联免疫吸附试验显示,在受试菌株中,KU15122 对 PGE2、IL-1β 和 IL-6 的抑制作用最为显著。此外,KU15122 还能抑制参与核因子-卡巴 B、激活蛋白-1 和丝裂原活化蛋白激酶通路的各种因子。此外,KU15122 还能减少活性氧的生成。KU15122的抗炎作用可能归因于细菌外多糖。总之,KU15122 对炎症性疾病具有抗炎潜力。
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来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
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