Long-term storage stability of PGL-I coated ELISA plates for anti-Mycobacterium leprae IgM detection

IF 1.7 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2024-07-02 DOI:10.1016/j.mimet.2024.106985

Matheus Bernardes Torres Fogaça , Djairo Pastor Saavedra , Maria Beatris de Jesus Sousa , Nicolle Kathlen Alves Belém de Oliveira , Mariane Martins de Araújo Stefani , Samira Buhrer-Sékula

引用次数: 0

Abstract

The assessment of ELISA plates coated with phenolic glycolipid-I/PGL-I revealed excellent stability during eight years of storage at room temperature, promoting consistent IgM antibody detection in multibacillary leprosy patients. These stable, standardized plates can significantly contribute to efficient leprosy serology research and support its widespread distribution and use in endemic countries.

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用于检测抗麻风分枝杆菌 IgM 的涂有 PGL-I 的 ELISA 板的长期储存稳定性。

对涂有酚类糖脂-I/PGL-I的酶联免疫吸附板进行的评估显示，在室温下保存八年期间，该板具有极佳的稳定性，从而促进了对多螨型麻风病人的 IgM 抗体检测的一致性。这些稳定的标准化平板可极大地促进麻风病血清学研究的效率，并支持其在麻风病流行国家的广泛传播和使用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.