Spartinivicinus poritis sp. nov., a red pigment-producing bacterium isolated from a scleractinian coral Porites lutea.

IF 2 3区 生物学 Q4 MICROBIOLOGY
Sumbal Sajid, Guoqiang Zhang, Zongyao Zhang, Yishan Lu, Lianguo Chen, Lin Cai
{"title":"<i>Spartinivicinus poritis</i> sp. nov., a red pigment-producing bacterium isolated from a scleractinian coral <i>Porites lutea</i>.","authors":"Sumbal Sajid, Guoqiang Zhang, Zongyao Zhang, Yishan Lu, Lianguo Chen, Lin Cai","doi":"10.1099/ijsem.0.006444","DOIUrl":null,"url":null,"abstract":"<p><p>A Gram-stain-negative, red pigment-producing, aerobic, and rod-shaped bacterial strain (A2-2<sup>T</sup>) was isolated from a bleached scleractinian coral (<i>Porites lutea</i>). Strain A2-2<sup>T</sup> grew with 1.0-7.0 % (w/v) NaCl (optimum, 3.0 %), at pH 6.0-11.0 (optimum, pH 8.0), and at 18-41 °C (optimum, 35 °C). Results of phylogenetic analysis based on 16S rRNA gene sequences suggested that strain A2-2<sup>T</sup> fell within the genus <i>Spartinivicinus</i> and was closely related to <i>Spartinivicinus ruber</i> S2-4-1H<sup>T</sup> (98.1 % sequence similarity) and <i>Spartinivicinus marinus</i> SM1973<sup>T</sup> (98.0 % sequence similarity). The predominant cellular fatty acids of strain A2-2<sup>T</sup> were C<sub>16 : 0</sub> (31.0 %), summed feature 3 (29.0 %), summed feature 8 (11.7 %), C<sub>12 : 0</sub> 3-OH (6.4 %), and C<sub>10 : 0</sub> 3-OH (5.5 %), while the major respiratory quinone was Q-9. The polar lipids mainly comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and an unidentified phospholipid. The genome size of strain A2-2<sup>T</sup> was 6.8 Mb, with a G+C content of 40.2 mol%. The DNA-DNA hybridization value was 24.2 % between A2-2<sup>T</sup> and <i>S. ruber</i> S2-4-1H<sup>T</sup> and 36.9 % between A2-2<sup>T</sup> and <i>S. marinus</i> SM1973<sup>T</sup>, while the average nucleotide identity values were 80.1 and 88.8 %, respectively. Based on these findings, strain A2-2<sup>T</sup> could be recognized to represent a novel species of the genus <i>Spartinivicinus</i>, for which the name <i>Spartinivicinus poritis</i> sp. nov. is proposed. The type strain is A2-2<sup>T</sup> (=MCCC 1K08228<sup>T</sup>=KCTC 8323<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"74 7","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of systematic and evolutionary microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006444","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

A Gram-stain-negative, red pigment-producing, aerobic, and rod-shaped bacterial strain (A2-2T) was isolated from a bleached scleractinian coral (Porites lutea). Strain A2-2T grew with 1.0-7.0 % (w/v) NaCl (optimum, 3.0 %), at pH 6.0-11.0 (optimum, pH 8.0), and at 18-41 °C (optimum, 35 °C). Results of phylogenetic analysis based on 16S rRNA gene sequences suggested that strain A2-2T fell within the genus Spartinivicinus and was closely related to Spartinivicinus ruber S2-4-1HT (98.1 % sequence similarity) and Spartinivicinus marinus SM1973T (98.0 % sequence similarity). The predominant cellular fatty acids of strain A2-2T were C16 : 0 (31.0 %), summed feature 3 (29.0 %), summed feature 8 (11.7 %), C12 : 0 3-OH (6.4 %), and C10 : 0 3-OH (5.5 %), while the major respiratory quinone was Q-9. The polar lipids mainly comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and an unidentified phospholipid. The genome size of strain A2-2T was 6.8 Mb, with a G+C content of 40.2 mol%. The DNA-DNA hybridization value was 24.2 % between A2-2T and S. ruber S2-4-1HT and 36.9 % between A2-2T and S. marinus SM1973T, while the average nucleotide identity values were 80.1 and 88.8 %, respectively. Based on these findings, strain A2-2T could be recognized to represent a novel species of the genus Spartinivicinus, for which the name Spartinivicinus poritis sp. nov. is proposed. The type strain is A2-2T (=MCCC 1K08228T=KCTC 8323T).

Spartinivicinus poritis sp.
从白化硬骨珊瑚(Porites lutea)中分离出一种革兰氏染色阴性、产红色素、需氧、杆状细菌菌株(A2-2T)。菌株 A2-2T 在 1.0-7.0 %(w/v)NaCl(最适 3.0 %)、pH 值 6.0-11.0 (最适 pH 值 8.0)和 18-41 ℃(最适 35 ℃)条件下生长。基于 16S rRNA 基因序列的系统进化分析结果表明,菌株 A2-2T 属于 Spartinivicinus 属,与 Spartinivicinus ruber S2-4-1HT (序列相似度为 98.1%)和 Spartinivicinus marinus SM1973T (序列相似度为 98.0%)亲缘关系密切。菌株 A2-2T 的主要细胞脂肪酸为 C16 :0 (31.0 %)、总特征 3 (29.0 %)、总特征 8 (11.7 %)、C12 :0 3-OH (6.4 %) 和 C10 :0 3-OH(6.4 %)和 C10 : 0 3-OH(5.5 %),而主要的呼吸醌是 Q-9。极性脂质主要包括磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油和一种不明磷脂。菌株 A2-2T 的基因组大小为 6.8 Mb,G+C 含量为 40.2 mol%。A2-2T与S. ruber S2-4-1HT的DNA-DNA杂交值为24.2%,与S. marinus SM1973T的DNA-DNA杂交值为36.9%,平均核苷酸同一性分别为80.1%和88.8%。基于这些发现,菌株 A2-2T 可被确认为 Spartinivicinus 属的一个新物种,并将其命名为 Spartinivicinus poritis sp.模式菌株为 A2-2T(=MCCC 1K08228T=KCTC 8323T)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
5.20
自引率
21.40%
发文量
426
审稿时长
1 months
期刊介绍: Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信