Intrahepatic distribution of nerves in Suncus murinus by whole-mount immunohistological observation.

IF 1.2 4区 医学 Q3 ANATOMY & MORPHOLOGY
Ke Ren, Xiangquan Chen, Ting Yang, Rujia Li, Shuang-Qin Yi
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引用次数: 0

Abstract

Background: In recent years, elucidating the actual state of the liver nervous system has attracted attention, owing to clinical needs, such as liver transplantation. Conventional methods for studying the intrahepatic nerve distribution mostly use liver tissue sections, specific markers for immunohistological studies, or anterograde/retrograde tracing in animals. However, knowledge of the three-dimensional structure of intrahepatic innervation is vague or speculative.

Materials and methods: In this study, Suncus murinus (Suncus) (n = 10) were perfused and fixed, the livers were excised, and the liver parenchyma was carefully removed, leaving only the intrahepatic vasculature. Specimens were prepared to study the three-dimensional structure of Suncus intrahepatic and hilar innervation by whole-mount immunohistochemical staining using a neurofilament protein antibody.

Results: After the nerves running along the intrahepatic arterial system entered the liver parenchyma from the hepatic hilum, they maintained a relatively rich distribution along the interlobular arteries until the distal end. The innervation of the portal system began to decrease after entering the liver parenchyma and decreased significantly after reaching the deep parts. By the time it reached the end of the interlobular vein, there was very little left. The number of nerves running along the intrahepatic bile duct system was significantly reduced after entering the porta hepatis, and innervation was difficult to observe after completely entering the liver parenchyma.

Conclusions: Whole-mount immunohistochemical analyses with an anti-NFP antibody showed that intrahepatic innervation mainly accompanied the hepatic interlobular arteries and extended to their terminal ends. Neuronal regulation is very important in the functional regulation of intrahepatic nutritional vessels. However, there were very few NFP-immunoreactive nerves accompanying the intrahepatic bile duct system, possibly suggesting that the functional regulation of the intrahepatic biliary system mainly relies on hormones and neuropeptides.

通过整片免疫组织学观察鼠尾葵神经在肝内的分布。
背景:近年来,由于肝移植等临床需要,阐明肝脏神经系统的实际状态引起了人们的关注。研究肝内神经分布的传统方法大多使用肝组织切片、免疫组织学研究的特异性标记物或动物的前向/后向追踪。然而,人们对肝内神经支配的三维结构的认识还很模糊,或者说是一种推测:在本研究中,对鼠太阳鸟(Suncus murinus)(n = 10)进行灌注和固定,切除肝脏,仔细去除肝实质,只留下肝内血管。制备标本后,使用神经丝蛋白抗体进行整片免疫组化染色,研究太阳穴肝内和肝门神经支配的三维结构:结果:沿肝内动脉系统运行的神经从肝门进入肝实质后,沿小叶间动脉一直到远端保持相对丰富的分布。门静脉系统的神经支配在进入肝实质后开始减少,到达深部后明显减少。当到达小叶间静脉末端时,已所剩无几。进入肝门后,沿肝内胆管系统运行的神经数量明显减少,完全进入肝实质后很难观察到神经支配:结论:用抗 NFP 抗体进行的整装免疫组化分析表明,肝内神经支配主要伴随肝小叶间动脉并延伸至其末端。神经元调控在肝内营养血管的功能调控中非常重要。然而,肝内胆管系统伴有极少的NFP免疫反应神经,这可能表明肝内胆管系统的功能调节主要依赖于激素和神经肽。
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来源期刊
Folia morphologica
Folia morphologica ANATOMY & MORPHOLOGY-
CiteScore
2.40
自引率
0.00%
发文量
218
审稿时长
6-12 weeks
期刊介绍: "Folia Morphologica" is an official journal of the Polish Anatomical Society (a Constituent Member of European Federation for Experimental Morphology - EFEM). It contains original articles and reviews on morphology in the broadest sense (descriptive, experimental, and methodological). Papers dealing with practical application of morphological research to clinical problems may also be considered. Full-length papers as well as short research notes can be submitted. Descriptive papers dealing with non-mammals, cannot be accepted for publication with some exception.
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