In mares resistant to endometrial infection, periovulatory treatment with ecbolic drugs does not influence uterine clearance or luteal development

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science Pub Date : 2024-07-01 DOI:10.1016/j.anireprosci.2024.107548

Younis Khan , Amr El-Shalofy , Martim Kaps , Camille Gautier , Christine Aurich

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引用次数: 0

Abstract

We aimed to determine associations between experimentally impaired uterine clearance or treatment with ecbolic drugs on luteal development in estrous mares after insemination. In a crossover design, eight mares were treated with saline (CON), clenbuterol (CLEN), oxytocin (OXY) and carbetocin (CARB) from the day of first insemination until 2 days after ovulation. Between treatments, the mares rested for one cycle. Estrous mares were examined for the presence of free intrauterine fluid by transrectal ultrasound. Endometrial swabs for cytology and bacteriology were collected on days 1 and 14. Blood samples were collected daily before AI until day 14 after ovulation for determination of progesterone and PGF_2α metabolites (PGFM). Differences between treatments were compared by a general linear model for repeated measures (SPSS 29). One mare was excluded because of a uterine infection in the control cycle. In all other mares, only minor amounts of free intrauterine fluid were present after insemination and decreased over time (P<0.05) with no treatment x time interaction. There was no effect of treatment on polymorphonucleated cells (PMN) in endometrial cytology after ovulation or PGFM secretion. Progesterone release from day 1–14 as well as pregnancy rate and conceptus size on day 14 was not influenced by treatment. In conclusion, treatment with clenbuterol does not impair uterine clearance in estrous mares resistant to endometritis. Repeated injection of the oxytocin analogue carbetocin during the early postovulatory period is not detrimental to corpus luteum function and can be recommended to enhance uterine clearance.

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在耐受子宫内膜感染的母马中，围排卵期使用蜕膜药物不会影响子宫清除或黄体发育。

我们的目的是确定实验性子宫清除受损或使用代谢药物治疗与发情母马受精后黄体发育之间的关系。在交叉设计中，八匹母马分别接受了生理盐水（CON）、盐酸克伦特罗（CLEN）、催产素（OXY）和卡贝菌素（CARB）治疗，治疗时间从首次人工授精当天开始，直至排卵后两天。在两次治疗之间，母马休息一个周期。通过经直肠超声波检查发情母马是否存在宫内游离液。第 1 天和第 14 天采集子宫内膜拭子进行细胞学和细菌学检查。每天在人工授精前至排卵后第 14 天采集血液样本，以测定孕酮和 PGF2α 代谢物 (PGFM)。采用重复测量的一般线性模型（SPSS 29）比较不同处理之间的差异。有一匹母马在对照周期中因子宫感染而被排除在外。在所有其他母马中，人工授精后仅有少量游离宫内液存在，并随着时间的推移而减少（P

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来源期刊

Animal Reproduction Science 农林科学-奶制品与动物科学

CiteScore

4.50

自引率

9.10%

发文量

136

审稿时长

54 days

期刊介绍： Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.