Evaluation of Anti-Biofilm and in Vitro Wound Healing Activity of Bacterial Cellulose Loaded with Nanoparticles and Borax

IF 4.7 3区 工程技术 Q2 ENGINEERING, ENVIRONMENTAL
Nur Bozbeyoglu Kart, Mine Sulak, Doğukan Mutlu, Volkan Kuzucu, Sevki Arslan, Nazime Mercan Dogan
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Abstract

Biofilms are a severe problem for public health because of the contributing recurrence of infections. Therefore, combating biofilms is a critical issue. In our study, we loaded zinc oxide (ZnO), zinc oxide borax (ZnOBorax), zinc copper oxide (ZnCuO2) nanoparticles and borax into bacterial cellulose (BC) to impart anti-biofilm and wound healing activity. The prepared BC loaded with nanoparticles (BC–NPs) was analysed via scanning electron microscopy. The nanoparticles’ geometric structure and placement in BC fibres were observed. We evaluated the biofilm inhibition and biofilm degradation activities of the BC–NPs against some pathogens via a crystal violet (CV) assay and XTT (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2 H-tetrazolium-5-carboxanilide) reduction assay. The effects of BC–NPs on cell proliferation and wound-healing ability were analysed in L929 cell line. BC–NPs exhibited better biofilm degradation activity than biofilm inhibition activity. According to the results of the CV assay, BC–ZnONPs, BC–Borax and BC–ZnOBoraxNPs inhibited 65.53%, 71.74% and 66.60% of biofilm formation of Staphylococcus aureus, respectively. BC–ZnCuO2NPs showed the most degradation activity on Pseudomonas aeruginosa and Listeria innocua biofilms. The XTT reduction assay results indicated a considerable reduction in the metabolic activity of the biofilms. Moreover, compared to the control group, BC loaded with borax and ZnO nanoparticle promoted cell migration without cytotoxicity.

Abstract Image

纳米颗粒和硼砂负载细菌纤维素的抗生物膜和体外伤口愈合活性评估
生物膜是一个严重的公共卫生问题,因为它会导致感染复发。因此,防治生物膜是一个关键问题。在我们的研究中,我们将氧化锌(ZnO)、氧化锌硼砂(ZnOBorax)、氧化锌铜(ZnCuO2)纳米颗粒和硼砂添加到细菌纤维素(BC)中,以赋予其抗生物膜和伤口愈合活性。通过扫描电子显微镜分析了制备的负载纳米颗粒(BC-NPs)的细菌纤维素。观察了纳米颗粒的几何结构和在 BC 纤维中的位置。我们通过水晶紫(CV)测定法和 XTT(2,3-双(2-甲氧基-4-硝基-5-磺酸苯基)-2 H-四唑-5-甲酰苯胺)还原测定法评估了 BC-NPs 对一些病原体的生物膜抑制和生物膜降解活性。在 L929 细胞系中分析了 BC-NPs 对细胞增殖和伤口愈合能力的影响。BC-NPs 的生物膜降解活性优于生物膜抑制活性。根据 CV 检测结果,BC-ZnONPs、BC-Borax 和 BC-ZnOBoraxNPs 对金黄色葡萄球菌生物膜形成的抑制率分别为 65.53%、71.74% 和 66.60%。BC-ZnCuO2NPs 对铜绿假单胞菌和无毒李斯特菌生物膜的降解活性最强。XTT 还原试验结果表明,生物膜的代谢活性大大降低。此外,与对照组相比,负载硼砂和氧化锌纳米粒子的 BC 可促进细胞迁移,且无细胞毒性。
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来源期刊
Journal of Polymers and the Environment
Journal of Polymers and the Environment 工程技术-高分子科学
CiteScore
9.50
自引率
7.50%
发文量
297
审稿时长
9 months
期刊介绍: The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.
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