{"title":"Evaluation of Anti-Biofilm and in Vitro Wound Healing Activity of Bacterial Cellulose Loaded with Nanoparticles and Borax","authors":"Nur Bozbeyoglu Kart, Mine Sulak, Doğukan Mutlu, Volkan Kuzucu, Sevki Arslan, Nazime Mercan Dogan","doi":"10.1007/s10924-024-03308-3","DOIUrl":null,"url":null,"abstract":"<div><p>Biofilms are a severe problem for public health because of the contributing recurrence of infections. Therefore, combating biofilms is a critical issue. In our study, we loaded zinc oxide (ZnO), zinc oxide borax (ZnOBorax), zinc copper oxide (ZnCuO<sub>2</sub>) nanoparticles and borax into bacterial cellulose (BC) to impart anti-biofilm and wound healing activity. The prepared BC loaded with nanoparticles (BC–NPs) was analysed via scanning electron microscopy. The nanoparticles’ geometric structure and placement in BC fibres were observed. We evaluated the biofilm inhibition and biofilm degradation activities of the BC–NPs against some pathogens via a crystal violet (CV) assay and XTT (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2 H-tetrazolium-5-carboxanilide) reduction assay. The effects of BC–NPs on cell proliferation and wound-healing ability were analysed in L929 cell line. BC–NPs exhibited better biofilm degradation activity than biofilm inhibition activity. According to the results of the CV assay, BC–ZnONPs, BC–Borax and BC–ZnOBoraxNPs inhibited 65.53%, 71.74% and 66.60% of biofilm formation of <i>Staphylococcus aureus</i>, respectively. BC–ZnCuO<sub>2</sub>NPs showed the most degradation activity on <i>Pseudomonas aeruginosa</i> and <i>Listeria innocua</i> biofilms. The XTT reduction assay results indicated a considerable reduction in the metabolic activity of the biofilms. Moreover, compared to the control group, BC loaded with borax and ZnO nanoparticle promoted cell migration without cytotoxicity.</p></div>","PeriodicalId":659,"journal":{"name":"Journal of Polymers and the Environment","volume":null,"pages":null},"PeriodicalIF":4.7000,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10924-024-03308-3.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Polymers and the Environment","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10924-024-03308-3","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
引用次数: 0
Abstract
Biofilms are a severe problem for public health because of the contributing recurrence of infections. Therefore, combating biofilms is a critical issue. In our study, we loaded zinc oxide (ZnO), zinc oxide borax (ZnOBorax), zinc copper oxide (ZnCuO2) nanoparticles and borax into bacterial cellulose (BC) to impart anti-biofilm and wound healing activity. The prepared BC loaded with nanoparticles (BC–NPs) was analysed via scanning electron microscopy. The nanoparticles’ geometric structure and placement in BC fibres were observed. We evaluated the biofilm inhibition and biofilm degradation activities of the BC–NPs against some pathogens via a crystal violet (CV) assay and XTT (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2 H-tetrazolium-5-carboxanilide) reduction assay. The effects of BC–NPs on cell proliferation and wound-healing ability were analysed in L929 cell line. BC–NPs exhibited better biofilm degradation activity than biofilm inhibition activity. According to the results of the CV assay, BC–ZnONPs, BC–Borax and BC–ZnOBoraxNPs inhibited 65.53%, 71.74% and 66.60% of biofilm formation of Staphylococcus aureus, respectively. BC–ZnCuO2NPs showed the most degradation activity on Pseudomonas aeruginosa and Listeria innocua biofilms. The XTT reduction assay results indicated a considerable reduction in the metabolic activity of the biofilms. Moreover, compared to the control group, BC loaded with borax and ZnO nanoparticle promoted cell migration without cytotoxicity.
期刊介绍:
The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.