Retrotransposon-mediated disruption of a chitin synthase gene confers insect resistance to Bacillus thuringiensis Vip3Aa toxin.

IF 9.8 1区生物学 Q1 Agricultural and Biological Sciences

PLoS Biology Pub Date : 2024-07-02 eCollection Date: 2024-07-01 DOI:10.1371/journal.pbio.3002704

Zhenxing Liu, Chongyu Liao, Luming Zou, Minghui Jin, Yinxue Shan, Yudong Quan, Hui Yao, Lei Zhang, Peng Wang, Zhuangzhuang Liu, Na Wang, Anjing Li, Kaiyu Liu, Bruce E Tabashnik, David G Heckel, Kongming Wu, Yutao Xiao

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引用次数: 0

Abstract

The vegetative insecticidal protein Vip3Aa from Bacillus thuringiensis (Bt) has been produced by transgenic crops to counter pest resistance to the widely used crystalline (Cry) insecticidal proteins from Bt. To proactively manage pest resistance, there is an urgent need to better understand the genetic basis of resistance to Vip3Aa, which has been largely unknown. We discovered that retrotransposon-mediated alternative splicing of a midgut-specific chitin synthase gene was associated with 5,560-fold resistance to Vip3Aa in a laboratory-selected strain of the fall armyworm, a globally important crop pest. The same mutation in this gene was also detected in a field population. Knockout of this gene via CRISPR/Cas9 caused high levels of resistance to Vip3Aa in fall armyworm and 2 other lepidopteran pests. The insights provided by these results could help to advance monitoring and management of pest resistance to Vip3Aa.

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逆转录转座子介导的几丁质合成酶基因干扰可使昆虫对苏云金芽孢杆菌 Vip3Aa 毒素产生抗性。

转基因作物产生了苏云金芽孢杆菌（Bt）的无性系杀虫蛋白 Vip3Aa，以对抗害虫对广泛使用的 Bt 结晶（Cry）杀虫蛋白的抗性。为了积极管理害虫的抗药性，迫切需要更好地了解对 Vip3Aa 产生抗性的遗传基础。我们发现，由逆转录转座子介导的中肠特异性几丁质合成酶基因的替代剪接与实验室选育的一种全球重要的农作物害虫--秋天军虫菌株对 Vip3Aa 产生的高达 5,560 倍的抗性有关。在田间种群中也检测到了该基因的相同突变。通过 CRISPR/Cas9 基因敲除该基因可使秋刺吸虫和其他两种鳞翅目害虫对 Vip3Aa 产生高水平的抗性。这些结果提供的启示有助于推进害虫对 Vip3Aa 抗性的监测和管理。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

PLoS Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-BIOLOGY

CiteScore

15.40

自引率

2.00%

发文量

359

审稿时长

3-8 weeks

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