PXL01 alters macrophage response with no effect on axonal outgrowth or Schwann cell response after nerve repair in rats.

IF 2.4 4区医学 Q4 CELL & TISSUE ENGINEERING

Regenerative medicine Pub Date : 2024-06-02 Epub Date: 2024-07-03 DOI:10.1080/17460751.2024.2361515

Derya Burcu Hazer Rosberg, Lena Stenberg, Margit Mahlapuu, Lars B Dahlin

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Abstract

Background: Adjunctive pharmacological treatment may improve nerve regeneration. We investigated nerve regeneration processes of PXL01 - a lactoferrin-derived peptide - after repair of the sciatic nerve in healthy Wistar rats.Materials & methods: PXL01, sodium hyaluronate (carrier) or sodium chloride was administered around the repair. After 6 days axonal outgrowth, Schwann cell response, pan- (CD68) and pro-healing (CD206) macrophages in sciatic nerve, sensory neuronal response in dorsal root ganglia (DRG) and expression of heat shock protein 27 (HSP27) in sciatic nerves and DRGs were analyzed.Results: Despite a lower number of pan-macrophages, other investigated variables in sciatic nerves or DRGs did not differ between the treatment groups.Conclusion: PLX01 applied locally inhibits inflammation through pan-macrophages in repaired sciatic nerves without any impact on nerve regeneration or pro-healing macrophages.

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PXL01 可改变巨噬细胞的反应，但对大鼠神经修复后的轴突生长或许旺细胞反应没有影响。

背景：辅助药物治疗可改善神经再生。我们研究了乳铁蛋白衍生肽 PXL01 在健康 Wistar 大鼠坐骨神经修复后的神经再生过程。材料与方法：在修复处周围注射 PXL01、透明质酸钠（载体）或氯化钠。6 天后，分析坐骨神经的轴突生长、许旺细胞反应、泛（CD68）和促愈合（CD206）巨噬细胞、背根神经节（DRG）的感觉神经元反应以及坐骨神经和 DRG 的热休克蛋白 27（HSP27）表达。结果：尽管泛巨噬细胞的数量较少，但坐骨神经或背根神经节的其他研究变量在治疗组之间没有差异。结论局部应用 PLX01 可通过修复的坐骨神经中的泛巨噬细胞抑制炎症，而不会影响神经再生或促进愈合的巨噬细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Regenerative medicine 医学-工程：生物医学

CiteScore

4.20

自引率

3.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Regenerative medicine replaces or regenerates human cells, tissue or organs, to restore or establish normal function*. Since 2006, Regenerative Medicine has been at the forefront of publishing the very best papers and reviews covering the entire regenerative medicine sector. The journal focusses on the entire spectrum of approaches to regenerative medicine, including small molecule drugs, biologics, biomaterials and tissue engineering, and cell and gene therapies – it’s all about regeneration and not a specific platform technology. The journal’s scope encompasses all aspects of the sector ranging from discovery research, through to clinical development, through to commercialization. Regenerative Medicine uniquely supports this important area of biomedical science and healthcare by providing a peer-reviewed journal totally committed to publishing the very best regenerative medicine research, clinical translation and commercialization. Regenerative Medicine provides a specialist forum to address the important challenges and advances in regenerative medicine, delivering this essential information in concise, clear and attractive article formats – vital to a rapidly growing, multidisciplinary and increasingly time-constrained community. Despite substantial developments in our knowledge and understanding of regeneration, the field is still in its infancy. However, progress is accelerating. The next few decades will see the discovery and development of transformative therapies for patients, and in some cases, even cures. Regenerative Medicine will continue to provide a critical overview of these advances as they progress, undergo clinical trials, and eventually become mainstream medicine.