Oluwafemi A Ojo, David Ajeigbe, Akingbolabo D Ogunlakin, Olalekan E Odesanmi, Mojisola Ayomipo, Godwin Berana, Peluola Ayeni, Omolola A Ajayi-Odoko, Damilare I Ayokunle, Adebola B Ojo, Basiru O Ajiboye, Omolara O Ojo, Samuel O Dahunsi
{"title":"Preclinical antidiabetic and antioxidant effects of <i>Erythrophleum africanum</i> (benth.) harms in streptozotocin-induced diabetic nephropathy.","authors":"Oluwafemi A Ojo, David Ajeigbe, Akingbolabo D Ogunlakin, Olalekan E Odesanmi, Mojisola Ayomipo, Godwin Berana, Peluola Ayeni, Omolola A Ajayi-Odoko, Damilare I Ayokunle, Adebola B Ojo, Basiru O Ajiboye, Omolara O Ojo, Samuel O Dahunsi","doi":"10.1515/jcim-2024-0090","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>This study investigated the antidiabetic effects of the methanolic extract of <i>E. africanum</i> (MEEA) stem bark on streptozotocin (STZ)-induced diabetic nephropathy (DN) in Wistar rats.</p><p><strong>Methods: </strong>The <i>in vitro</i> enzyme (α-amylase) inhibitory activity of MEEA was measured using a standard procedure. Diabetic rats with fasting blood glucose above 250 mg/dL were considered diabetic and were divided into the following groups: control (distilled water-treated), diabetic-control, diabetic metformin (100 mg/kg), diabetes + MEEA (150 mg/kg), and diabetes + MEEA (300 mg/kg) via oral gavage once daily for 14 days. At the end of the experimental period, kidney tissues were collected for biochemical and histological analyses. Kidney apoptosis and marker gene expression were measured by real-time quantitative PCR.</p><p><strong>Results: </strong>MEEA exhibited α-amylase inhibitory effects. MEEA significantly (p<0.05) reduced the STZ-induced increases in blood glucose, serum urea, serum creatinine, uric acid, alanine aminotransferase, alkaline phosphatase, and malondialdehyde and increased the STZ-induced decreases in superoxide dismutase, catalase, and reduced glutathione. In addition, MEEA protects against DN by significantly downregulating the mRNA expression of cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), cAMP-response binding protein (CREB), and cFOS and upregulating B-cell lymphoma 2 (Bcl-2), suggesting that the nephroprotective ability of MEEA is due to the modulation of the cAMP/PKA/CREB/cFOS signaling pathway. Furthermore, MEEA treatment protected against histopathological alterations observed in diabetic rats.</p><p><strong>Conclusions: </strong>The data from this study suggest that MEEA modulates glucose homeostasis and inhibits redox imbalance in DN rats.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":"349-359"},"PeriodicalIF":0.0000,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Complementary and Integrative Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/jcim-2024-0090","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
Abstract
Objectives: This study investigated the antidiabetic effects of the methanolic extract of E. africanum (MEEA) stem bark on streptozotocin (STZ)-induced diabetic nephropathy (DN) in Wistar rats.
Methods: The in vitro enzyme (α-amylase) inhibitory activity of MEEA was measured using a standard procedure. Diabetic rats with fasting blood glucose above 250 mg/dL were considered diabetic and were divided into the following groups: control (distilled water-treated), diabetic-control, diabetic metformin (100 mg/kg), diabetes + MEEA (150 mg/kg), and diabetes + MEEA (300 mg/kg) via oral gavage once daily for 14 days. At the end of the experimental period, kidney tissues were collected for biochemical and histological analyses. Kidney apoptosis and marker gene expression were measured by real-time quantitative PCR.
Results: MEEA exhibited α-amylase inhibitory effects. MEEA significantly (p<0.05) reduced the STZ-induced increases in blood glucose, serum urea, serum creatinine, uric acid, alanine aminotransferase, alkaline phosphatase, and malondialdehyde and increased the STZ-induced decreases in superoxide dismutase, catalase, and reduced glutathione. In addition, MEEA protects against DN by significantly downregulating the mRNA expression of cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), cAMP-response binding protein (CREB), and cFOS and upregulating B-cell lymphoma 2 (Bcl-2), suggesting that the nephroprotective ability of MEEA is due to the modulation of the cAMP/PKA/CREB/cFOS signaling pathway. Furthermore, MEEA treatment protected against histopathological alterations observed in diabetic rats.
Conclusions: The data from this study suggest that MEEA modulates glucose homeostasis and inhibits redox imbalance in DN rats.
期刊介绍：
Journal of Complementary and Integrative Medicine (JCIM) focuses on evidence concerning the efficacy and safety of complementary medical (CM) whole systems, practices, interventions and natural health products, including herbal and traditional medicines. The journal is edited by Ed Lui of the University of Western Ontario. Topics: -Quality, efficacy, and safety of natural health products, dietary supplements, traditional medicines and their synthetic duplicates -Efficacy and safety of complementary therapies -Evidence-based medicine and practice, including evidence of traditional use -Curriculum development, educational system and competency of complementary health programs -Methodologies on research and evaluation of traditional medicines and herbal products -Integrative medicine: basic and clinical research and practice -Innovation in CAM Curriculum -Educational Material Design
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
