Effects of Renal Denervation on Ouabain-Induced Hypertension in Rats.

IF 1.9 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE
International Journal of Hypertension Pub Date : 2024-06-24 eCollection Date: 2024-01-01 DOI:10.1155/2024/4763189
Minna Tang, Jialu Hu, Wenshu Li, Ningzhi Zhang, Sisi Ning, Yan Yan, Zhaoqiang Cui
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Abstract

Background: Ouabain, a Na+, K+-ATPase inhibitor, is elevated in hypertensive patients. Evidence suggests ouabain contributes to hypertension mainly through activation of the sympathetic nervous system (SNS). Renal nerves play a vital role in the regulation of SNS activity, so we hypothesize that renal denervation may attenuate the development of ouabain-induced hypertension.

Methods and results: Forty Sprague-Dawley rats were divided into following groups (n = 10 each): control group (sham surgery plus intraperitoneal saline injection), RDN group (renal denervation (RDN) plus intraperitoneal saline injection), ouabain group (sham surgery plus intraperitoneal ouabain injection), and ouabain + RDN group (RDN plus intraperitoneal ouabain injection). After eight weeks, compared with the control group, rats in the ouabain group exhibited elevated blood pressure (P < 0.05), increased plasma epinephrine, norepinephrine, angiotensin II, and aldosterone levels (P < 0.05). These indexes could be significantly ameliorated by RDN. RDN also reduced the thickening of aortic tunica media and downregulated the expression of proliferating cell nuclear antigen (PCNA) in the thoracic aorta induced by ouabain. Masson staining and echocardiography showed that myocardial fibrosis and increased left ventricular mass in the ouabain group could be attenuated by RDN.

Conclusions: The present study reveals that renal nerves play an important role in the development of ouabain-induced hypertension. RDN could inhibit the pressor effect and the myocardial remodeling induced by ouabain potentially via inhibiting catecholamine release and vascular smooth muscle cell proliferation. Clinical studies are needed to explore whether RDN may exhibit better antihypertensive effects on hypertensive patients with high plasma ouabain levels as compared to those with normal plasma ouabain levels.

肾脏去神经对大鼠欧巴因诱发高血压的影响
背景:欧巴马因是一种 Na+、K+-ATPase 抑制剂,在高血压患者中会升高。有证据表明,欧贝因主要通过激活交感神经系统(SNS)导致高血压。肾脏神经在交感神经系统活动的调节中起着至关重要的作用,因此我们假设肾脏去神经化可能会减轻欧巴马因诱发的高血压的发展:将 40 只 Sprague-Dawley 大鼠分为以下几组(每组 10 只):对照组(假手术加腹腔注射生理盐水)、RDN 组(肾脏去神经化(RDN)加腹腔注射生理盐水)、乌巴因组(假手术加腹腔注射乌巴因)和乌巴因 + RDN 组(RDN 加腹腔注射乌巴因)。八周后,与对照组相比,乌巴因组大鼠血压升高(P < 0.05),血浆肾上腺素、去甲肾上腺素、血管紧张素 II 和醛固酮水平升高(P < 0.05)。RDN 可明显改善这些指标。RDN 还能减少主动脉中膜的增厚,并下调乌巴因诱导的胸主动脉增殖细胞核抗原(PCNA)的表达。Masson染色和超声心动图显示,RDN可减轻欧贝因组心肌纤维化和左心室质量增加:结论:本研究揭示了肾脏神经在欧阿巴因诱导的高血压发病过程中起着重要作用。结论:本研究揭示了肾脏神经在乌巴因诱导的高血压发病过程中起着重要作用,肾脏神经可通过抑制儿茶酚胺释放和血管平滑肌细胞增殖,抑制乌巴因诱导的加压效应和心肌重构。与血浆中欧巴因水平正常的高血压患者相比,RDN 是否能对血浆中欧巴因水平较高的高血压患者产生更好的降压效果,还需要临床研究来探讨。
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来源期刊
International Journal of Hypertension
International Journal of Hypertension Medicine-Internal Medicine
CiteScore
4.00
自引率
5.30%
发文量
45
期刊介绍: International Journal of Hypertension is a peer-reviewed, Open Access journal that provides a forum for clinicians and basic scientists interested in blood pressure regulation and pathophysiology, as well as treatment and prevention of hypertension. The journal publishes original research articles, review articles, and clinical studies on the etiology and risk factors of hypertension, with a special focus on vascular biology, epidemiology, pediatric hypertension, and hypertensive nephropathy.
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