HLA-E-expressing macrophage polarization and increased NKG2A/CD94 expression in adult-onset Still's disease.

IF 3.3 4区 医学 Q3 IMMUNOLOGY
Immunologic Research Pub Date : 2024-10-01 Epub Date: 2024-07-03 DOI:10.1007/s12026-024-09512-6
Yasuhiro Shimojima, Takanori Ichikawa, Dai Kishida, Ryota Takamatsu, Yoshiki Sekijima
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引用次数: 0

Abstract

We investigated the phenotypic characteristics of human leukocyte antigen (HLA)-E-expressing macrophages, NKG2A/CD94 expression in T and natural killer (NK) cells, and their interactions in patients with adult-onset Still's disease (AOSD). Peripheral blood mononuclear cells from 22 patients with AOSD and 22 healthy controls (HC) were used. Isolated monocytes were cultured first with macrophage colony-stimulating factor to differentiate into M0 macrophages and subsequently with lipopolysaccharide/interferon-γ or interleukin-4 to differentiate into M1 or M2 macrophages, respectively. HLA-E and NKG2A/CD94 expression levels were evaluated using quantitative RT-PCR and flow cytometry. HLA-E expression in M0 and M2 macrophages was significantly higher in patients with AOSD than in HC, and was positively correlated with serum C-reactive protein levels and erythrocyte sedimentation rate. NKG2A/CD94 expression in CD4 + and CD8 + T cells was significantly higher in patients with AOSD than in HC, but that in NK cells was not significantly different. In patients with AOSD, NKG2A expression in CD4 + T cells positively correlated with HLA-E expression in M0, M1, and M2 macrophages. CD94 expression in CD8 + T cells inversely correlated with HLA-E expression in M1 and M2 macrophages. NKG2A and CD94 expression in NK cells inversely correlated with HLA-E expression in M0, M1, and M2 macrophages. No significant correlation was observed between HLA-E and NKG2A/CD94 expression in HC. Increased expression of HLA-E in macrophages and NKG2A/CD94 in T cells can be observed in the inflammatory condition of AOSD. HLA-E-expressing macrophages may be associated with NKG2A/CD94 expression in T and NK cells with different correlations.

Abstract Image

成人型斯蒂尔病的 HLA-E 表达巨噬细胞极化和 NKG2A/CD94 表达增加。
我们研究了人类白细胞抗原(HLA)-E 表达巨噬细胞的表型特征、T 细胞和自然杀伤细胞(NK)中 NKG2A/CD94 的表达以及它们在成人型斯蒂尔病(AOSD)患者中的相互作用。研究使用了 22 名 AOSD 患者和 22 名健康对照组(HC)的外周血单核细胞。分离出的单核细胞先用巨噬细胞集落刺激因子培养分化成M0巨噬细胞,然后用脂多糖/干扰素-γ或白细胞介素-4分别培养分化成M1或M2巨噬细胞。采用定量 RT-PCR 和流式细胞术评估了 HLA-E 和 NKG2A/CD94 的表达水平。AOSD患者M0和M2巨噬细胞中的HLA-E表达明显高于HC患者,且与血清C反应蛋白水平和红细胞沉降率呈正相关。AOSD 患者 CD4 + 和 CD8 + T 细胞中的 NKG2A/CD94 表达明显高于 HC 患者,但 NK 细胞中的 NKG2A/CD94 表达无明显差异。在 AOSD 患者中,CD4 + T 细胞中 NKG2A 的表达与 M0、M1 和 M2 巨噬细胞中 HLA-E 的表达呈正相关。CD8 + T细胞中CD94的表达与M1和M2巨噬细胞中HLA-E的表达成反比。NK 细胞中 NKG2A 和 CD94 的表达与 M0、M1 和 M2 巨噬细胞中 HLA-E 的表达成反比。在 HC 中,HLA-E 和 NKG2A/CD94 的表达没有明显的相关性。在 AOSD 的炎症状态下,可以观察到巨噬细胞中 HLA-E 和 T 细胞中 NKG2A/CD94 的表达增加。HLA-E表达的巨噬细胞可能与T细胞和NK细胞中NKG2A/CD94的表达存在不同的相关性。
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来源期刊
Immunologic Research
Immunologic Research 医学-免疫学
CiteScore
6.90
自引率
0.00%
发文量
83
审稿时长
6-12 weeks
期刊介绍: IMMUNOLOGIC RESEARCH represents a unique medium for the presentation, interpretation, and clarification of complex scientific data. Information is presented in the form of interpretive synthesis reviews, original research articles, symposia, editorials, and theoretical essays. The scope of coverage extends to cellular immunology, immunogenetics, molecular and structural immunology, immunoregulation and autoimmunity, immunopathology, tumor immunology, host defense and microbial immunity, including viral immunology, immunohematology, mucosal immunity, complement, transplantation immunology, clinical immunology, neuroimmunology, immunoendocrinology, immunotoxicology, translational immunology, and history of immunology.
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