Berberine protects against sepsis-related acute lung injury in rats via PPAR-γ signaling pathway upregulation and improvement at the cellular level: Functional, biochemical, and immunohistochemistry study.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-01 DOI:10.4149/gpb_2024008

Mohamed M Khalifa, Nermeen A Bastawy, Laila A Rashed, Hanan A Hassan, Omnia M Abdel-Maksoud, Fatma E Hassan

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Abstract

This study aimed to assess the prophylactic effects of Berberine on experimentally induced lung sepsis and examine its effects on selected cytokines, genes, and protein expression besides the histopathological evaluation. Berberine significantly reduced the wet/dry lung ratio, the broncho-alveolar lavage fluid (BALF) protein, cells, neutrophils percentage, and cytokines levels. In addition, pretreatment with Berberine decreased the myeloperoxidase (MPO) and malondialdehyde (MDA) levels and decreased gene expression of nuclear factor kappa B (NF-κB), monocyte chemoattractant protein-1 (MCP-1), and the intracellular adhesion molecule 1 (ICAM-1) by RT-qPCR analysis, revealing Berberine's antioxidant and anti-inflammatory mode of action. Western blot analysis revealed increased peroxisome proliferator-activated receptor gamma (PPAR-γ) expression in the Berberine pretreated group compared to the cecal ligation and puncture (CLP) group, in which the histopathological examination evidenced this improvement. In conclusion, Berberine improved lung sepsis via its PPAR-γ mediated antioxidant and anti-inflammatory effects.

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小檗碱通过 PPAR-γ 信号通路的上调和细胞水平的改善保护大鼠免受脓毒症相关急性肺损伤：功能、生化和免疫组化研究。

本研究旨在评估小檗碱对实验性肺败血症的预防作用，并检查其对特定细胞因子、基因和蛋白质表达的影响，以及组织病理学评估。小檗碱能明显降低肺干湿比、支气管肺泡灌洗液（BALF）蛋白、细胞、中性粒细胞百分比和细胞因子水平。此外，小檗碱还能降低髓过氧化物酶（MPO）和丙二醛（MDA）水平，并通过 RT-qPCR 分析降低核因子卡巴 B（NF-κB）、单核细胞趋化蛋白-1（MCP-1）和细胞内粘附分子 1（ICAM-1）的基因表达，揭示了小檗碱的抗氧化和抗炎作用模式。Western 印迹分析显示，与盲肠结扎和穿刺（CLP）组相比，小檗碱预处理组的过氧化物酶体增殖激活受体γ（PPAR-γ）表达增加，组织病理学检查也证明了这一改善。总之，小檗碱通过 PPAR-γ 介导的抗氧化和抗炎作用改善了肺败血症。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.