Overexpression of insulin-like growth factor-2 mRNA-binding protein 1 is associated with periodontal disease

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2024-07-02 DOI:10.1016/j.jobcr.2024.06.001

Burra Anand Deepika , Jaiganesh Ramamurthy , Balachander Kannan , Vijayashree Priyadharsini Jayaseelan , Paramasivam Arumugam

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引用次数: 0

Abstract

Objective

To investigate the potential role of a novel m6A RNA regulator, Insulin-like Growth Factor-2 mRNA-binding protein 1 (IGF2BP1), in periodontal disease pathogenesis.

Materials and methods

Gingival tissue samples from 60 periodontitis patients and 60 healthy individuals were analyzed for IGF2BP1 mRNA and protein expression via real-time quantitative PCR (RT-qPCR) and Western blotting. Additionally, Porphyromonas gingivalis Lipopolysaccharide (Pg-LPS) -induced human gingival fibroblasts (HGFs) were evaluated for IGF2BP1 and proinflammatory cytokine expression. In silico functional analysis further explored potential molecular mechanisms.

Results

IGF2BP1 mRNA and protein levels were significantly higher in the periodontitis group compared to the healthy group. Functional analysis implicated IGF2BP1 in regulating the IL-17 signaling pathway, a key player in inflammation. Pg-LPS treatment upregulated IGF2BP1 and proinflammatory cytokines in HGFs, supporting this finding.

Conclusion

Our study suggests that IGF2BP1 overexpression contributes to periodontitis pathogenesis, potentially through IL-17 signaling. Further research is needed to elucidate the precise molecular mechanisms and explore IGF2BP1 as a potential therapeutic target or biomarker for this common oral disease.

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胰岛素样生长因子-2 mRNA 结合蛋白 1 的过表达与牙周病有关

材料与方法通过实时定量 PCR（RT-qPCR）和 Western 印迹分析 60 例牙周炎患者和 60 例健康人的牙龈组织样本的 IGF2BP1 mRNA 和蛋白表达。此外，还评估了牙龈卟啉菌脂多糖（Pg-LPS）诱导的人牙龈成纤维细胞（HGFs）的 IGF2BP1 和促炎细胞因子表达情况。结果牙周炎组的 IGF2BP1 mRNA 和蛋白水平明显高于健康组。功能分析表明，IGF2BP1参与调节IL-17信号通路，而IL-17是炎症的关键因素。我们的研究表明，IGF2BP1 的过度表达可能是通过 IL-17 信号传导导致牙周炎发病的。需要进一步的研究来阐明精确的分子机制，并探索 IGF2BP1 作为这种常见口腔疾病的潜在治疗靶点或生物标志物。

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.